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小鼠D-乳酸脱氢酶酶免试剂盒,(D-LDH)ELISA检测试剂盒
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更新时间:2016-10-14  |  阅读:1033

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本产品仅用于科研,不用于临床

小鼠D-乳酸脱氢酶酶免试剂盒,(D-LDH)ELISA检测试剂盒 ,英文名:D-LDH ELISA Kit ,英文缩写:D-LDH ,常见试剂盒规格:96T/48T,产品别名:小鼠D-乳酸脱氢酶ELISA检测试剂盒,D-LDH 检测试剂盒, D-LDH 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠D-乳酸脱氢酶酶免试剂盒,(D-LDH)ELISA检测试剂盒 在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。

TNF受体关联因子6(TRAF6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for TNF Receptor Associated Factor 6 (TRAF6)48T/96TY13285Homo sapiens (Human)
旁血小板溶蛋白(PPL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Periplakin (PPL)48T/96TY13286Mus musculus (Mouse)
淋巴细胞抗原96(LY96)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lymphocyte Antigen 96 (LY96)48T/96TY13287Homo sapiens (Human)
Diaphanous同源物1(DIAPH1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Diaphanous Homolog 1 (DIAPH1)48T/96TY13288Homo sapiens (Human)
解整合素金属蛋白酶22(ADAM22)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for A Disintegrin And Metalloprotease 22 (ADAM22)48T/96TY13289Homo sapiens (Human)
电子转移黄素蛋白α肽(ETFα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Electron Transfer Flavoprotein Alpha Polypeptide (ETFa)48T/96TY13290Homo sapiens (Human)
精氨基琥珀酸合成酶1(ASS1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Argininosuccinate Synthetase 1 (ASS1)48T/96TY13291Homo sapiens (Human)
蛋白二硫化物异构酶A6(PDIA6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Disulfide Isomerase A6 (PDIA6)48T/96TY13292Homo sapiens (Human)
半胱氨酸蛋白酶抑制剂6(CST6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cystatin 6 (CST6)48T/96TY13293Homo sapiens (Human)
促泌素(SCGN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secretagogin (SCGN)48T/96TY13294Rattus norvegicus (Rat)
促泌素(SCGN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secretagogin (SCGN)48T/96TY13295Mus musculus (Mouse)
赖氨酰氧化酶样蛋白4(LOXL4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lysyl Oxidase Like Protein 4 (LOXL4)48T/96TY13296Homo sapiens (Human)
内皮细胞特异分子1(ESM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Endothelial Cell Specific Molecule 1 (ESM1)48T/96TY13297Homo sapiens (Human)
金属硫蛋白1E(MT1E)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Metallothionein 1E (MT1E)48T/96TY13298Homo sapiens (Human)
凝血因子Ⅵ(F6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Coagulation Factor VI (F6)48T/96TY13299Rattus norvegicus (Rat)
无翅型MMTV整合位点家族成员4(WNT4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 4 (WNT4)48T/96TY13300Rattus norvegicus (Rat)
USP6氨基端样蛋白(USP6NL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for USP6 N-Terminal Like Protein (USP6NL)48T/96TY13301Homo sapiens (Human)
血幼素(HJV)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hemojuvelin (HJV)48T/96TY13302Rattus norvegicus (Rat)
盐皮质激素受体(MR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mineralocorticoid Receptor (MR)48T/96TY13303Rattus norvegicus (Rat)
AMPA离子能谷氨酸受体1(GRIA1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1)48T/96TY13304Rattus norvegicus (Rat)
N-甲基-D-天氡氨酸离子能谷氨酸受体1(GRIN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1)48T/96TY13305Rattus norvegicus (Rat)
杀菌性/通透性增加蛋白(BPI)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bactericidal/Permeability Increasing Protein (BPI)48T/96TY13306Mus musculus (Mouse)
骨骼肌肌动蛋白α1(ACTα1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Actin Alpha 1, Skeletal Muscle (ACTa1)48T/96TY13307Rattus norvegicus (Rat)
骨骼肌慢肌肌钙蛋白T(TNNT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Troponin T Type 1, Slow Skeletal (TNNT1)48T/96TY13308Homo sapiens (Human)
细胞色素P450家族成员1B1(CYP1B1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytochrome P450 1B1 (CYP1B1)48T/96TY13309Homo sapiens (Human)
肌球蛋白重链6(MYH6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)48T/96TY13310Rattus norvegicus (Rat)
丝裂原激活蛋白激酶激酶1(MAP2K1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mitogen Activated Protein Kinase Kinase 1 (MAP2K1)48T/96TY13311Homo sapiens (Human)
血小板反应蛋白2(THBS2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Thrombospondin 2 (THBS2)48T/96TY13312Homo sapiens (Human)
肝配蛋白A受体10(EPHA10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ephrin Type A Receptor 10 (EPHA10)48T/96TY13313Homo sapiens (Human)
转化受体电位阳离子通道亚家族M成员6(TRPM6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transient Receptor Potential Cation Channel Subfamily M, Member 6 (TRPM6)48T/96TY13314Rattus norvegicus (Rat)
转化受体电位阳离子通道亚家族M成员7(TRPM7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transient Receptor Potential Cation Channel Subfamily M, Member 7 (TRPM7)48T/96TY13315Rattus norvegicus (Rat)
转化受体电位阳离子通道亚家族V成员1(TRPV1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1)48T/96TY13316Mus musculus (Mouse)
色胺酸羟化酶2(TPH2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tryptophan Hydroxylase 2 (TPH2)48T/96TY13317Mus musculus (Mouse)
RNA聚合酶转录终止因子Ⅰ(TTF1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transcription Termination Factor, RNA polymerase I (TTF1)48T/96TY13318Homo sapiens (Human)
Podocan蛋白(PODN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Podocan (PODN)48T/96TY13319Mus musculus (Mouse)
生长因子受体结合蛋白10(Grb10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Growth Factor Receptor Bound Protein 10 (Grb10)48T/96TY13320Homo sapiens (Human)
胰岛素样生长因子2-mRNA结合蛋白3(IGF2BP3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3)48T/96TY13321Homo sapiens (Human)
黏病毒耐药蛋白1(MX1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myxovirus Resistance 1 (MX1)48T/96TY13322Homo sapiens (Human)
酶原粒蛋白16同源物B(ZG16B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B)48T/96TY13323Homo sapiens (Human)
甘露糖受体C1样蛋白1(MRC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mannose Receptor C Type 1 Like Protein 1(MRC1)48T/96TY13324Homo sapiens (Human)
糖蛋白Ⅴ(GP5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glycoprotein V, Plaet (GP5)48T/96TY13325Homo sapiens (Human)
白介素10受体α(IL10Rα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 10 Receptor Alpha (IL10Ra)48T/96TY13326Rattus norvegicus (Rat)
白介素10受体β(IL10Rβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 10 Receptor Beta (IL10Rb)48T/96TY13327Rattus norvegicus (Rat)
巢蛋白(NID)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nidogen (NID)48T/96TY13328Homo sapiens (Human)
静止素Q6硫基氧化酶1(QSOX1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1)48T/96TY13329Homo sapiens (Human)
连接桥粒斑珠蛋白(JUP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Junction Plakoglobin (JUP)48T/96TY13330Homo sapiens (Human)
抗增生抗体靶分子1(TAPA1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Target Of The Antiproliferative Antibody 1 (TAPA1)48T/96TY13331Homo sapiens (Human)
细胞外基质磷酸糖蛋白(MEPE)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Matrix Extracellular Phosphoglycoprotein (MEPE)48T/96TY13332Mus musculus (Mouse)
苯酚磺基转移酶(PST)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phenol Sulfotransferase (PST)48T/96TY13333Mus musculus (Mouse)
凝血因子Ⅶ(F7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Coagulation Factor VII (F7)48T/96TY13334Mus musculus (Mouse)
各种类型ELISA测定时,   一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。

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