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小鼠c-jun ELISA检测试剂盒
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更新时间:2016-10-16  |  阅读:873

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小鼠c-jun ELISA检测试剂盒 ,英文名:小鼠c-jun ELISA试剂盒 ,英文缩写:小鼠c-jun ELISA试剂盒 ,常见试剂盒规格:96T/48T,产品别名:小鼠c-jun ELISA试剂盒 小鼠c-junELISA试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠c-jun ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。

再生胰岛衍生蛋白1β(REG1β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Regenerating Islet Derived Protein 1 Beta (REG1b)48T/96TY13035Homo sapiens (Human)
赖氨酰氧化酶样蛋白2(LOXL2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lysyl Oxidase Like Protein 2 (LOXL2)48T/96TY13036Homo sapiens (Human)
钙同线蛋白2(CLSTN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calsyntenin 2 (CLSTN2)48T/96TY13037Homo sapiens (Human)
甘露糖结合凝集素2(LMAN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lectin, Mannose Binding 2 (LMAN2)48T/96TY13038Homo sapiens (Human)
钙调宁蛋白1(CNN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calponin 1, Basic (CNN1)48T/96TY13039Homo sapiens (Human)
蛋白Z(PROZ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Z (PROZ)48T/96TY13040Mus musculus (Mouse)
促肾上腺皮质素释放激素结合蛋白(CRHBP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Corticotropin Releasing Hormone Binding Protein (CRHBP)48T/96TY13041Homo sapiens (Human)
WNT1诱导信号通道蛋白1(WISP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for WNT1 Inducible Signaling Pathway Protein 1 (WISP1)48T/96TY13042Mus musculus (Mouse)
内皮素2(EDN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Endothelin 2 (EDN2)48T/96TY13043Rattus norvegicus (Rat)
血管舒张剂激活磷蛋白(VASP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vasodilator Stimulated Phosphoprotein (VASP)48T/96TY13044Homo sapiens (Human)
神经疾病靶标酯酶(NTE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neuropathy Target Esterase (NTE)48T/96TY13045Rattus norvegicus (Rat)
整合素αV(ITGαV)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Integrin Alpha V (ITGaV)48T/96TY13046Homo sapiens (Human)
基质重塑关联蛋白5(MXRA5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Remodelling Associated Protein 5 (MXRA5)48T/96TY13047Homo sapiens (Human)
斯钙素1(STC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Stanniocalcin 1 (STC1)48T/96TY13048Mus musculus (Mouse)
Klotho蛋白(KL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Klotho (KL)48T/96TY13049Rattus norvegicus (Rat)
层黏连蛋白受体1(LAMR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Laminin Receptor 1 (LAMR1)48T/96TY13050Homo sapiens (Human)
心营养素样细胞因子1(CLCF1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cardiotrophin Like Cytokine Factor 1 (CLCF1)48T/96TY13051Homo sapiens (Human)
细胞骨架活性调节蛋白(ARC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Activity Regulated Cytoskeleton Associated Protein (ARC)48T/96TY13052Mus musculus (Mouse)
生长分化因子3(GDF3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Growth Differentiation Factor 3 (GDF3)48T/96TY13053Mus musculus (Mouse)
生长分化因子3(GDF3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Growth Differentiation Factor 3 (GDF3)48T/96TY13054Rattus norvegicus (Rat)
多巴胺转运蛋白(DAT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dopamine Transporter (DAT)48T/96TY13055Homo sapiens (Human)
异柠檬酸脱氢酶1(IDH1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Isocitrate Dehydrogenase 1, Soluble (IDH1)48T/96TY13056Homo sapiens (Human)
骨髓基质细胞抗原2(BST2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Marrow Stromal Cell Antigen 2 (BST2)48T/96TY13057Homo sapiens (Human)
膜铁转运蛋白(FPN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ferroportin (FPN)48T/96TY13058Mus musculus (Mouse)
Ⅳ型胶原α3(COL4α3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Collagen Type IV Alpha 3 (COL4a3)48T/96TY13059Homo sapiens (Human)
原钙黏素15(PCDH15)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protocadherin 15 (PCDH15)48T/96TY13060Homo sapiens (Human)
铜伴侣超氧化物歧化酶(SOD4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Superoxide Dismutase, Copper Chaperone (SOD4)48T/96TY13061Rattus norvegicus (Rat)
过氧化还原酶1(PRDX1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Peroxiredoxin 1 (PRDX1)48T/96TY13062Homo sapiens (Human)
外生骨疣样蛋白1(EXTL1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Exostoses Like Protein 1 (EXTL1)48T/96TY13063Homo sapiens (Human)
RAD23同源物B(RAD23B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for RAD23 Homolog B (RAD23B)48T/96TY13064Homo sapiens (Human)
苄氯素1(BECN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Beclin 1 (BECN1)48T/96TY13065Homo sapiens (Human)
催乳素诱导蛋白(PIP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prolactin Induced Protein (PIP)48T/96TY13066Homo sapiens (Human)
R-脊椎蛋白3(RSPO3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for R-Spondin 3 (RSPO3)48T/96TY13067Homo sapiens (Human)
干扰素α21(IFNα21)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Alpha 21 (IFNa21)48T/96TY13068Homo sapiens (Human)
唾液酸乙酰酯酶(SIAE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sialic Acid Acetylesterase (SIAE)48T/96TY13069Homo sapiens (Human)
音猬因子(SHH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hedgehog Homolog, Sonic (SHH)48T/96TY13070Rattus norvegicus (Rat)
印度刺猬因子(IHH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hedgehog Homolog, Indian (IHH)48T/96TY13071Rattus norvegicus (Rat)
胰岛素样蛋白6(INSL6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Protein 6 (INSL6)48T/96TY13072Mus musculus (Mouse)
尿皮质素2(UCN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Urocortin 2 (UCN2)48T/96TY13073Rattus norvegicus (Rat)
尿皮质素3(UCN3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Urocortin 3 (UCN3)48T/96TY13074Rattus norvegicus (Rat)
组织蛋白酶G(CTSG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cathepsin G (CTSG)48T/96TY13075Mus musculus (Mouse)
膜联蛋白A1(ANXA1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Annexin A1 (ANXA1)48T/96TY13076Mus musculus (Mouse)
白蛋白启动子D位点结合蛋白(DBP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for D Site Of Albumin Promoter Binding Protein (DBP)48T/96TY13077Rattus norvegicus (Rat)
V-Yes-1 Yamaguchi肉瘤病毒相关癌基因同源物(LYN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN)48T/96TY13078Homo sapiens (Human)
神经生长因子诱导蛋白B(NGFIB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nerve Growth Factor IB (NGFIB)48T/96TY13079Rattus norvegicus (Rat)
生长因子受体结合蛋白2(Grb2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Growth Factor Receptor Bound Protein 2 (Grb2)48T/96TY13080Homo sapiens (Human)
毛状样蛋白1(COTL1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Coactosin Like Protein 1 (COTL1)48T/96TY13081Homo sapiens (Human)
腓骨蛋白7(FBLN7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibulin 7 (FBLN7)48T/96TY13082Mus musculus (Mouse)
溶质载体家族1成员5(SLC1A5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Solute Carrier Family 1, Member 5 (SLC1A5)48T/96TY13083Homo sapiens (Human)
P物质(SP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Substance P (SP)48T/96TY13084Canis familiaris; Canine (Dog)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。

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