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小鼠解整合素样金属蛋白酶9酶免试剂盒,(ADAM9)ELISA检测试剂盒 ,英文名:ADAM9 ELISA Kit ,英文缩写:ADAM9 ,常见试剂盒规格:96T/48T,产品别名:小鼠解整合素样金属蛋白酶9ELISA检测试剂盒,ADAM9 检测试剂盒, ADAM9 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠解整合素样金属蛋白酶9酶免试剂盒,(ADAM9)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
皮质醇结合球蛋白(CBG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Corticosteroid Binding Globulin (CBG)48T/96TY12785Mus musculus (Mouse)
防御素α1(DEFα1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Defensin Alpha 1, Neutrophil (DEFa1)48T/96TY12786Mus musculus (Mouse)
四旋蛋白30(TSPAN30)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tetraspanin 30 (TSPAN30)48T/96TY12787Mus musculus (Mouse)
细胞色素P450家族成员1A1(CYP1A1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytochrome P450 1A1 (CYP1A1)48T/96TY12788Homo sapiens (Human)
雌激素受体相关受体α(ERRα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Estrogen Related Receptor Alpha (ERRa)48T/96TY12789Homo sapiens (Human)
极低密度脂蛋白受体(VLDLR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Very Low Density Lipoprotein Receptor (VLDLR)48T/96TY12790Rattus norvegicus (Rat)
淀粉样前体蛋白(APP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Amyloid Precursor Protein (APP)48T/96TY12791Rattus norvegicus (Rat)
E1A结合蛋白P300(EP300)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for E1A Binding Protein P300 (EP300)48T/96TY12792Homo sapiens (Human)
周期素依赖性激酶抑制因子1A(CDKN1A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cyclin Dependent Kinase Inhibitor 1A (CDKN1A)48T/96TY12793Rattus norvegicus (Rat)
层黏连蛋白α5(LAMα5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Laminin Alpha 5 (LAMa5)48T/96TY12794Homo sapiens (Human)
神经调节素2(NRG2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neuregulin 2 (NRG2)48T/96TY12795Homo sapiens (Human)
水通道蛋白8(AQP8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aquaporin 8 (AQP8)48T/96TY12796Homo sapiens (Human)
双特异性磷酸酶9(DUSP9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dual Specificity Phosphatase 9 (DUSP9)48T/96TY12797Homo sapiens (Human)
神经源性分化蛋白6(NEUROD6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neurogenic Differentiation 6 (NEUROD6)48T/96TY12798Homo sapiens (Human)
血小板内皮细胞粘附分子1(PECAM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plaet/Endothelial Cell Adhesion Molecule (PECAM1)48T/96TY12799Mus musculus (Mouse)
血小板反应蛋白2(THBS2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Thrombospondin 2 (THBS2)48T/96TY12800Mus musculus (Mouse)
碳酸酐酶Ⅸ(CA9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Carbonic Anhydrase IX (CA9)48T/96TY12801Rattus norvegicus (Rat)
白介素1受体辅助蛋白(IL1RAP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Accessory Protein (IL1RAP)48T/96TY12802Mus musculus (Mouse)
磷酸肌醇依赖性蛋白激酶1(PDPK1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phosphoinositide Dependent Protein Kinase 1 (PDPK1)48T/96TY12803Mus musculus (Mouse)
精胺氧化酶(SMOX)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Spermine Oxidase (SMOX)48T/96TY12804Homo sapiens (Human)
HtrA丝氨酸肽酶4(HTRA4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for HtrA Serine Peptidase 4 (HTRA4)48T/96TY12805Homo sapiens (Human)
阻抑素(PHB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prohibitin (PHB)48T/96TY12806Rattus norvegicus (Rat)
果糖二磷酸醛缩酶A(ALDOA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aldolase A, Fructose Bisphosphate (ALDOA)48T/96TY12807Rattus norvegicus (Rat)
Ⅵ型胶原α1(COL6α1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Collagen Type VI Alpha 1 (COL6a1)48T/96TY12808Mus musculus (Mouse)
果糖二磷酸醛缩酶B(ALDOB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aldolase B, Fructose Bisphosphate (ALDOB)48T/96TY12809Rattus norvegicus (Rat)
果糖二磷酸醛缩酶C(ALDOC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aldolase C, Fructose Bisphosphate (ALDOC)48T/96TY12810Rattus norvegicus (Rat)
谷氨酰胺合成酶(GS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutamine synthetase (GS)48T/96TY12811Mus musculus (Mouse)
肝配蛋白B2(EFNB2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ephrin B2 (EFNB2)48T/96TY12812Homo sapiens (Human)
溶质载体家族30成员8(SLC30A8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Solute Carrier Family 30 Member 8 (SLC30A8)48T/96TY12813Homo sapiens (Human)
半胱氨酸丰富蛋白1(CRIP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cysteine Rich Protein 1, Intestinal (CRIP1)48T/96TY12814Homo sapiens (Human)
谷氨酰胺合成酶(GS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutamine synthetase (GS)48T/96TY12815Rattus norvegicus (Rat)
CD83分子(CD83)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 83 (CD83)48T/96TY12816Mus musculus (Mouse)
激素敏感性脂肪酶(LIPE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lipase, Hormone Sensitive (LIPE)48T/96TY12817Rattus norvegicus (Rat)
信号素7A(SEMA7A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Semaphorin 7A (SEMA7A)48T/96TY12818Mus musculus (Mouse)
亚铁螯合酶(FECH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ferrochelatase (FECH)48T/96TY12819Homo sapiens (Human)
N-乙酰半乳糖胺酶α(NAGα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for N-Acetylgalactosaminidase Alpha (NAGa)48T/96TY12820Mus musculus (Mouse)
ⅣD组磷脂酶A2(PLA2G4D)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipase A2, Group IVD (PLA2G4D)48T/96TY12821Homo sapiens (Human)
补体成分5a(C5a)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Complement Component 5a (C5a)48T/96TY12822Canis familiaris; Canine (Dog)
YY肽(PYY)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Peptide YY (PYY)48T/96TY12823Mus musculus (Mouse)
线粒体超氧化物歧化酶(SOD2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Superoxide Dismutase 2, Mitochondrial (SOD2)48T/96TY12824Rattus norvegicus (Rat)
中性鞘磷脂酶(NSMASE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neutral Sphingomyelinase (NSMASE)48T/96TY12825Rattus norvegicus (Rat)
沉默调节蛋白1(SIRT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sirtuin 1 (SIRT1)48T/96TY12826Rattus norvegicus (Rat)
沉默调节蛋白3(SIRT3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sirtuin 3 (SIRT3)48T/96TY12827Rattus norvegicus (Rat)
过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARγC1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a)48T/96TY12828Rattus norvegicus (Rat)
脱氧核糖核酸酶Ⅰ(DNASE1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Deoxyribonuclease I (DNASE1)48T/96TY12829Mus musculus (Mouse)
胆碱乙酰转移酶(ChAT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Choline Acetyltransferase (ChAT)48T/96TY12830Mus musculus (Mouse)
Ⅻ型胶原(COL12)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Collagen Type XII (COL12)48T/96TY12831Homo sapiens (Human)
低密度脂蛋白受体相关蛋白6(LRP6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Low Density Lipoprotein Receptor Related Protein 6 (LRP6)48T/96TY12832Homo sapiens (Human)
尿调蛋白(UMOD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Uromodulin (UMOD)48T/96TY12833Mus musculus (Mouse)
肝配蛋白B2(EFNB2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ephrin B2 (EFNB2)48T/96TY12834Mus musculus (Mouse)
各种类型ELISA测定时, 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。