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小鼠抗核抗体酶免试剂盒,(ANA)ELISA检测试剂盒 ,英文名:ANA ELISA Kit ,英文缩写:ANA ,常见试剂盒规格:96T/48T,产品别名:小鼠抗核抗体ELISA检测试剂盒,ANA 检测试剂盒, ANA 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠抗核抗体酶免试剂盒,(ANA)ELISA检测试剂盒 在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
分拣蛋白1(SORT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sortilin 1 (SORT1)48T/96TY12685Homo sapiens (Human)
淀粉样蛋白β前体样蛋白2(APLP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Amyloid Beta Precursor Like Protein 2 (APLP2)48T/96TY12686Homo sapiens (Human)
钙离子转运ATP酶A2(ATP2A2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2)48T/96TY12687Homo sapiens (Human)
尿调蛋白(UMOD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Uromodulin (UMOD)48T/96TY12688Rattus norvegicus (Rat)
S100钙结合蛋白A8(S100A8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for S100 Calcium Binding Protein A8 (S100A8)48T/96TY12689Mus musculus (Mouse)
S100钙结合蛋白A9(S100A9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for S100 Calcium Binding Protein A9 (S100A9)48T/96TY12690Mus musculus (Mouse)
蛋白Z依赖性蛋白酶抑制因子(ZPI)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Z Dependent Protease Inhibitor (ZPI)48T/96TY12691Homo sapiens (Human)
赖氨酰氧化酶(LOX)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lysyl Oxidase (LOX)48T/96TY12692Homo sapiens (Human)
胞裂蛋白6(SEPT6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Septin 6 (SEPT6)48T/96TY12693Homo sapiens (Human)
蛋氨酸腺苷转移酶Ⅱα(MAT2α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Methionine Adenosyltransferase II Alpha (MAT2a)48T/96TY12694Homo sapiens (Human)
GPI锚定分子样蛋白(GML)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML)48T/96TY12695Homo sapiens (Human)
B-细胞κ-轻肽基因增强子抑制因子激酶β(IκBKβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb)48T/96TY12696Mus musculus (Mouse)
核糖体蛋白L23A(RPL23A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ribosomal Protein L23A (RPL23A)48T/96TY12697Homo sapiens (Human)
同线蛋白2(ST2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Syntenin 2 (ST2)48T/96TY12698Homo sapiens (Human)
Endoglin蛋白(ENG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Endoglin (ENG)48T/96TY12699Canis familiaris; Canine (Dog)
CD19分子(CD19)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 19 (CD19)48T/96TY12700Rattus norvegicus (Rat)
钾中间小电导钙激活通道蛋白亚家族N成员2(KCNN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2)48T/96TY12701Homo sapiens (Human)
白介素1家族成员9(IL1F9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Family, Member 9 (IL1F9)48T/96TY12702Homo sapiens (Human)
增殖关联蛋白2G4(PA2G4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Proliferation Associated Protein 2G4 (PA2G4)48T/96TY12703Homo sapiens (Human)
类固醇5α还原酶1(SRD5α1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Steroid 5 Alpha Reductase 1 (SRD5a1)48T/96TY12704Rattus norvegicus (Rat)
膜联蛋白A4(ANXA4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Annexin A4 (ANXA4)48T/96TY12705Homo sapiens (Human)
角蛋白9(KRT9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Keratin 9 (KRT9)48T/96TY12706Mus musculus (Mouse)
多功能蛋白聚糖(VS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Versican (VS)48T/96TY12707Mus musculus (Mouse)
密封蛋白(OCLN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Occludin (OCLN)48T/96TY12708Mus musculus (Mouse)
分泌型卷曲相关蛋白1(SFRP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secreted Frizzled Related Protein 1 (SFRP1)48T/96TY12709Homo sapiens (Human)
运动因子相关蛋白1(MRP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Motility Related Protein (MRP1)48T/96TY12710Homo sapiens (Human)
骨成型蛋白3(BMP3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 3 (BMP3)48T/96TY12711Homo sapiens (Human)
非受体酪氨酸激酶2(TNK2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tyrosine Kinase, Non Receptor 2 (TNK2)48T/96TY12712Homo sapiens (Human)
封闭蛋白2(CLDN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Claudin 2 (CLDN2)48T/96TY12713Mus musculus (Mouse)
肌钯蛋白(MYPN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myopalladin (MYPN)48T/96TY12714Homo sapiens (Human)
血管非炎性蛋白1(VNN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vanin 1 (VNN1)48T/96TY12715Rattus norvegicus (Rat)
鸟苷酸环化酶激活因子2A(GUCA2A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Guanylate Cyclase Activator 2A (GUCA2A)48T/96TY12716Homo sapiens (Human)
GM2神经节苷脂激活因子(GM2A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for GM2 Ganglioside Activator (GM2A)48T/96TY12717Homo sapiens (Human)
溶酶体酸性磷酸酶2(ACP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Acid Phosphatase 2, Lysosomal (ACP2)48T/96TY12718Homo sapiens (Human)
溶酶体酸性磷酸酶2(ACP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Acid Phosphatase 2, Lysosomal (ACP2)48T/96TY12719Mus musculus (Mouse)
组织蛋白酶D(CTSD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cathepsin D (CTSD)48T/96TY12720Homo sapiens (Human)
组织蛋白酶D(CTSD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cathepsin D (CTSD)48T/96TY12721Mus musculus (Mouse)
精氨酸酶(Arg)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Arginase (Arg)48T/96TY12722Mus musculus (Mouse)
Discs大同源物4(DLG4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Discs, Large Homolog 4 (DLG4)48T/96TY12723Rattus norvegicus (Rat)
中性鞘磷脂酶(NSMASE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neutral Sphingomyelinase (NSMASE)48T/96TY12724Homo sapiens (Human)
抑制素βE(INHβE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Inhibin Beta E (INHbE)48T/96TY12725Homo sapiens (Human)
DEAD框肽5(DDX5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for DEAD Box Polypeptide 5 (DDX5)48T/96TY12726Homo sapiens (Human)
乳铁传递蛋白(LTF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactoferrin (LTF)48T/96TY12727Sus scrofa; Porcine (Pig)
蛋白二硫化物异构酶A2(PDIA2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Disulfide Isomerase A2 (PDIA2)48T/96TY12728Mus musculus (Mouse)
蛋白二硫化物异构酶A5(PDIA5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Disulfide Isomerase A5 (PDIA5)48T/96TY12729Homo sapiens (Human)
核小体装配蛋白1样1(NAP1L1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1)48T/96TY12730Homo sapiens (Human)
妊娠带蛋白(PZP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pregnancy Zone Protein (PZP)48T/96TY12731Homo sapiens (Human)
精氨酸/丝氨酸丰富剪接因子2(SRSF2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Serine/Arginine Rich Splicing Factor 2 (SRSF2)48T/96TY12732Homo sapiens (Human)
醛脱氢酶1家族成员A1(ALDH1A1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1)48T/96TY12733Homo sapiens (Human)
前列腺蛋白类脂肪酶B(LIPB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lipophilin B, Prostatein Like (LIPB)48T/96TY12734Homo sapiens (Human)
各种类型ELISA测定时, 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。