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小鼠Ⅰ型胶原C端肽酶免试剂盒,(CTX-Ⅰ)ELISA检测试剂盒 ,英文名:CTX-Ⅰ ELISA Kit ,英文缩写:CTX-Ⅰ ,常见试剂盒规格:96T/48T,产品别名:小鼠Ⅰ型胶原C端肽ELISA检测试剂盒,CTX-Ⅰ 检测试剂盒, CTX-Ⅰ 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠Ⅰ型胶原C端肽酶免试剂盒,(CTX-Ⅰ)ELISA检测试剂盒 在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
干扰素调节因子9(IRF9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Regulatory Factor 9 (IRF9)48T/96TY12585Homo sapiens (Human)
分泌型卷曲相关蛋白1(SFRP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secreted Frizzled Related Protein 1 (SFRP1)48T/96TY12586Mus musculus (Mouse)
整合素β1(ITGβ1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Integrin Beta 1 (ITGb1)48T/96TY12587Homo sapiens (Human)
脂肪酸转运蛋白5(FATP5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fatty Acid Transport Protein 5 (FATP5)48T/96TY12588Homo sapiens (Human)
血管生成素样蛋白3(ANGPTL3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiopoietin Like Protein 3 (ANGPTL3)48T/96TY12589Mus musculus (Mouse)
亮氨酸丰富重复LGI家族成员3(LGI3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leucine Rich Repeat LGI Family, Member 3 (LGI3)48T/96TY12590Homo sapiens (Human)
多聚蛋白1(MMRN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Multimerin 1 (MMRN1)48T/96TY12591Homo sapiens (Human)
低氧诱导因子2α(HIF2α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hypoxia Inducible Factor 2 Alpha (HIF2a)48T/96TY12592Homo sapiens (Human)
低氧诱导因子2α(HIF2α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hypoxia Inducible Factor 2 Alpha (HIF2a)48T/96TY12593Mus musculus (Mouse)
醛脱氢酶1家族成员A2(ALDH1A2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2)48T/96TY12594Homo sapiens (Human)
内酰胺酶β(LACTβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactamase Beta (LACTb)48T/96TY12595Homo sapiens (Human)
无翅型MMTV整合位点家族成员5A(WNT5A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)48T/96TY12596Homo sapiens (Human)
解整合素金属蛋白酶12(ADAM12)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for A Disintegrin And Metalloprotease 12 (ADAM12)48T/96TY12597Homo sapiens (Human)
趋化因子C-X3-C-基元受体1(CX3CR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Chemokine C-X3-C-Motif Receptor 1 (CX3CR1)48T/96TY12598Mus musculus (Mouse)
107kDa肌醇多聚磷酸-4-磷酸酶Ⅰ型(INPP4A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A)48T/96TY12599Homo sapiens (Human)
107kDa肌醇多聚磷酸-4-磷酸酶Ⅰ型(INPP4A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A)48T/96TY12600Mus musculus (Mouse)
层粘连蛋白β3(LAMβ3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Laminin Beta 3 (LAMb3)48T/96TY12601Homo sapiens (Human)
融合蛋白(FUS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fusion (FUS)48T/96TY12602Mus musculus (Mouse)
黑素转铁蛋白(MFI2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Melanotransferrin (MFI2)48T/96TY12603Homo sapiens (Human)
视神经病变诱导反应蛋白(OPTN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Optineurin (OPTN)48T/96TY12604Mus musculus (Mouse)
8-羟基鸟嘌呤糖苷酶1(OGG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Oxoguanine Glycosylase 1 (OGG1)48T/96TY12605Homo sapiens (Human)
尿嘧啶DNA糖基化酶(UNG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Uracil DNA Glycosylase (UNG)48T/96TY12606Homo sapiens (Human)
胸苷嘧啶DNA糖基化酶(TDG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Thymine DNA Glycosylase (TDG)48T/96TY12607Homo sapiens (Human)
单链选择性单功能尿嘧啶DNA糖基化酶1(SMUG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1)48T/96TY12608Homo sapiens (Human)
N-甲基嘌呤DNA糖基化酶(MPG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for N-Methylpurine DNA Glycosylase (MPG)48T/96TY12609Homo sapiens (Human)
MutY同源物(MUTYH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for MutY Homolog (MUTYH)48T/96TY12610Homo sapiens (Human)
激活蛋白C(APC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Activated Protein C (APC)48T/96TY12611Sus scrofa; Porcine (Pig)
激活蛋白C(APC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Activated Protein C (APC)48T/96TY12612Rattus norvegicus (Rat)
嗜铬蛋白B(CHGB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Chromogranin B (CHGB)48T/96TY12613Homo sapiens (Human)
肾酶(RNLS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Renalase (RNLS)48T/96TY12614Homo sapiens (Human)
原钙黏素15(PCDH15)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protocadherin 15 (PCDH15)48T/96TY12615Mus musculus (Mouse)
RAD51同源物(RAD51)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for RAD51 Homolog (RAD51)48T/96TY12616Homo sapiens (Human)
黑素瘤抑制性活性蛋白1(MIA1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Melanoma Inhibitory Activity Protein 1 (MIA1)48T/96TY12617Homo sapiens (Human)
极低密度脂蛋白(VLDL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Very Low Density Lipoprotein (VLDL)48T/96TY12618Mus musculus (Mouse)
儿茶酚-O-甲基转移酶(COMT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Catechol-O-Methyltransferase (COMT)48T/96TY12619Homo sapiens (Human)
苯酚磺基转移酶(PST)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phenol Sulfotransferase (PST)48T/96TY12620Homo sapiens (Human)
孕烷X受体(PXR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pregnane X Receptor (PXR)48T/96TY12621Homo sapiens (Human)
骨骼肌特异性受体酪氨酸激酶(MUSK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK)48T/96TY12622Homo sapiens (Human)
糖化血红蛋白(HbA1C)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glycated Hemoglobin (HbA1C)48T/96TY12623Homo sapiens (Human)
抑肽酶(AP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aprotinin (AP)48T/96TY12624Bos taurus; Bovine (Cattle)
皮质醇结合球蛋白(CBG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Corticosteroid Binding Globulin (CBG)48T/96TY12625Rattus norvegicus (Rat)
FK506结合蛋白5(FKBP5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for FK506 Binding Protein 5 (FKBP5)48T/96TY12626Homo sapiens (Human)
单酰甘油-O-酰基转移酶2(MOGAT2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Monoacylglycerol-O-acyltransferase 2 (MOGAT2)48T/96TY12627Homo sapiens (Human)
二酰甘油-O-酰基转移酶同源物2(DGAT2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2)48T/96TY12628Homo sapiens (Human)
二酰甘油-O-酰基转移酶同源物1(DGAT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1)48T/96TY12629Homo sapiens (Human)
白细胞衍生趋化因子1(LECT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leukocyte Cell Derived Chemotaxin 1 (LECT1)48T/96TY12630Mus musculus (Mouse)
足盂蛋白(PCX)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Podocalyxin (PCX)48T/96TY12631Mus musculus (Mouse)
夏科雷登氏结晶蛋白(CLC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Charcot Leyden Crystal Protein (CLC)48T/96TY12632Mus musculus (Mouse)
ⅫB组磷脂酶A2(PLA2G12B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipase A2, Group XIIB (PLA2G12B)48T/96TY12633Homo sapiens (Human)
DNA甲基转移酶3B(DNMT3B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for DNA Methyltransferase 3B (DNMT3B)48T/96TY12634Mus musculus (Mouse)
各种类型ELISA测定时, 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。