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小鼠丝氨酸/苏氨酸蛋白磷酸酶酶免试剂盒,(STK)ELISA检测试剂盒 ,英文名:STK ELISA Kit ,英文缩写:STK ,常见试剂盒规格:96T/48T,产品别名:小鼠丝氨酸/苏氨酸蛋白磷酸酶ELISA检测试剂盒,STK 检测试剂盒, STK 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠丝氨酸/苏氨酸蛋白磷酸酶酶免试剂盒,(STK)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
成纤维细胞生长因子受体3(FGFR3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor Receptor 3 (FGFR3)48T/96TY12485Homo sapiens (Human)
成纤维细胞生长因子受体4(FGFR4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor Receptor 4 (FGFR4)48T/96TY12486Homo sapiens (Human)
皮肤衍生肽酶抑制因子3(PI3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Peptidase Inhibitor 3, Skin Derived (PI3)48T/96TY12487Homo sapiens (Human)
Ⅶ型胶原(COL7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Collagen Type VII (COL7)48T/96TY12488Homo sapiens (Human)
白介素17C(IL17C)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 17C (IL17C)48T/96TY12489Mus musculus (Mouse)
转铁蛋白受体(TFR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transferrin Receptor (TFR)48T/96TY12490Rattus norvegicus (Rat)
整合素β3(ITGβ3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Integrin Beta 3 (ITGb3)48T/96TY12491Homo sapiens (Human)
肿瘤坏死因子配体超家族成员14(TNFSF14)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14)48T/96TY12492Mus musculus (Mouse)
基质金属蛋白酶19(MMP19)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 19 (MMP19)48T/96TY12493Mus musculus (Mouse)
骨形成蛋白8B(BMP8B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 8B (BMP8B)48T/96TY12494Mus musculus (Mouse)
胰岛素样蛋白5(INSL5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Protein 5 (INSL5)48T/96TY12495Mus musculus (Mouse)
N-乙酰α-D-氨基葡萄糖苷酶(NAGLU)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for N-Acetyl Alpha-D-Glucosaminidase (NAGLU)48T/96TY12496Homo sapiens (Human)
尿皮质素2(UCN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Urocortin 2 (UCN2)48T/96TY12497Homo sapiens (Human)
层粘连蛋白γ2(LAMγ2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Laminin Gamma 2 (LAMC2)48T/96TY12498Homo sapiens (Human)
钙周期素结合蛋白(CACYBP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calcyclin Binding Protein (CACYBP)48T/96TY12499Homo sapiens (Human)
癌胚抗原相关细胞粘附分子1(CEACAM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Carcinoembryonic Antigen Related Cell Adhesion Molecule 1 (CEACAM1)48T/96TY12500Homo sapiens (Human)
胰腺型磷脂酶A2(pPLA2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipase A2, Pancreas (pPLA2)48T/96TY12501Sus scrofa; Porcine (Pig)
Corin蛋白(CRN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Corin (CRN)48T/96TY12502Homo sapiens (Human)
Rho GDP解离抑制因子α(ARHGDIα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Rho GDP Dissociation Inhibitor Alpha (ARHGDIa)48T/96TY12503Homo sapiens (Human)
胞裂蛋白5(SEPT5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Septin 5 (SEPT5)48T/96TY12504Homo sapiens (Human)
胃动素(MTL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Motilin (MTL)48T/96TY12505Rattus norvegicus (Rat)
组织转谷氨酰胺酶(TGM2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transglutaminase 2, Tissue (TGM2)48T/96TY12506Rattus norvegicus (Rat)
补体成分1r(C1r)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Complement Component 1, R Subcomponent (C1r)48T/96TY12507Homo sapiens (Human)
补体成分1s(C1s)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Complement Component 1, S Subcomponent (C1s)48T/96TY12508Homo sapiens (Human)
微管关联丝/苏氨酸激酶2(MAST2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Microtubule Associated Serine/Threonine Kinase 2 (MAST2)48T/96TY12509Homo sapiens (Human)
快肌肌钙蛋白C(TNNC2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Troponin C Type 2, Fast (TNNC2)48T/96TY12510Homo sapiens (Human)
弹性蛋白(ELN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Elastin (ELN)48T/96TY12511Mus musculus (Mouse)
丙酮酸脱氢酶β(PDHβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pyruvate Dehydrogenase Beta (PDHb)48T/96TY12512Mus musculus (Mouse)
赖氨酸tRNA合成酶(KARS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lysyl tRNA Synthetase (KARS)48T/96TY12513Homo sapiens (Human)
赖氨酸tRNA合成酶(KARS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lysyl tRNA Synthetase (KARS)48T/96TY12514Mus musculus (Mouse)
酮戊二酸脱氢酶(OGDH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Oxoglutarate Dehydrogenase (OGDH)48T/96TY12515Homo sapiens (Human)
钙网蛋白(CRT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calreticulin (CRT)48T/96TY12516Mus musculus (Mouse)
CD83分子(CD83)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 83 (CD83)48T/96TY12517Homo sapiens (Human)
组织蛋白酶B(CTSB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cathepsin B (CTSB)48T/96TY12518Homo sapiens (Human)
外核苷酸焦磷酸酶/磷酸二酯酶2(ENPP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2)48T/96TY12519Homo sapiens (Human)
白介素18结合蛋白(IL18BP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 18 Binding Protein (IL18BP)48T/96TY12520Mus musculus (Mouse)
卵磷脂胆固醇脂酰转移酶(LCAT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lecithin Cholesterol Acyltransferase (LCAT)48T/96TY12521Homo sapiens (Human)
支链α-酮酸脱氢酶(BCKDK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK)48T/96TY12522Rattus norvegicus (Rat)
白介素19(IL19)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 19 (IL19)48T/96TY12523Homo sapiens (Human)
花生四烯酸-5-脂加氧酶(ALOX5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Arachidonate-5-Lipoxygenase (ALOX5)48T/96TY12524Mus musculus (Mouse)
血管生成素样蛋白2(ANGPTL2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiopoietin Like Protein 2 (ANGPTL2)48T/96TY12525Mus musculus (Mouse)
细胞色素P450氧化还原酶(CPR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytochrome P450 Reductase (CPR)48T/96TY12526Rattus norvegicus (Rat)
C-型凝集素域家族3成员B(CLEC3B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for C-Type Lectin Domain Family 3, Member B (CLEC3B)48T/96TY12527Homo sapiens (Human)
神经元正五聚蛋白Ⅱ(NPTX2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neuronal Pentraxin II (NPTX2)48T/96TY12528Homo sapiens (Human)
免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Immunoglobulin G (IgG)48T/96TY12529Bos taurus; Bovine (Cattle)
促胰液素(SCT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secretin (SCT)48T/96TY12530Mus musculus (Mouse)
骨成型蛋白10(BMP10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 10 (BMP10)48T/96TY12531Homo sapiens (Human)
自身免疫调节因子(AIRE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Autoimmune Regulator (AIRE)48T/96TY12532Homo sapiens (Human)
5羟色胺转运蛋白(SERT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Serotonin Transporter (SERT)48T/96TY12533Homo sapiens (Human)
组织蛋白酶G(CTSG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cathepsin G (CTSG)48T/96TY12534Rattus norvegicus (Rat)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。