当前位置:首页 > 产品展示 > ELISA检测试剂盒 > 小鼠ELISA检测试剂盒 > 小鼠抗小核糖核蛋白/Sm抗体酶免试剂盒,(snRNP/Sm)ELISA检测试剂盒
详情介绍
本产品仅用于科研,不用于临床
小鼠抗小核糖核蛋白/Sm抗体酶免试剂盒,(snRNP/Sm)ELISA检测试剂盒 ,英文名:snRNP/Sm ELISA Kit ,英文缩写:snRNP/Sm ,常见试剂盒规格:96T/48T,产品别名:小鼠抗小核糖核蛋白/Sm抗体ELISA检测试剂盒,snRNP/Sm 检测试剂盒, snRNP/Sm 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠抗小核糖核蛋白/Sm抗体酶免试剂盒,(snRNP/Sm)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
生长分化因子5(GDF5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Growth Differentiation Factor 5 (GDF5)48T/96TY12435Homo sapiens (Human)
羧肽酶E(CPE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Carboxypeptidase E (CPE)48T/96TY12436Homo sapiens (Human)
白介素23受体(IL23R)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 23 Receptor (IL23R)48T/96TY12437Homo sapiens (Human)
TATA框结合蛋白关联因子12(TAF12)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for TATA Box Binding Protein Associated Factor 12 (TAF12)48T/96TY12438Mus musculus (Mouse)
精氨酸/丝氨酸丰富剪接因子4(SRSF4))检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Serine/Arginine Rich Splicing Factor 4 (SRSF4)48T/96TY12439Mus musculus (Mouse)
蛋白磷酸酶3调节因子亚基1(PPP3R1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1)48T/96TY12440Mus musculus (Mouse)
N-甲基-D-天氡氨酸离子能谷氨酸受体2A(GRIN2A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A (GRIN2A)48T/96TY12441Mus musculus (Mouse)
组氨酸丰富糖蛋白(HRG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Histidine Rich Glycoprotein (HRG)48T/96TY12442Mus musculus (Mouse)
p53上调凋亡调节因子(PUMA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for p53 Upregulated Modulator Of Apoptosis (PUMA)48T/96TY12443Rattus norvegicus (Rat)
T-细胞可诱导共刺激分子(ICOS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Inducible T-Cell Co Stimulator (ICOS)48T/96TY12444Homo sapiens (Human)
分泌型卷曲相关蛋白5(SFRP5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secreted Frizzled Related Protein 5 (SFRP5)48T/96TY12445Homo sapiens (Human)
重肽铁蛋白(FTH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ferritin, Heavy Polypeptide (FTH)48T/96TY12446Homo sapiens (Human)
2',5'-寡腺苷酸合成酶1(OAS1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for 2',5'-Oligoadenylate Synthetase 1 (OAS1)48T/96TY12447Homo sapiens (Human)
白介素22受体α2(IL2Rα2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 22 Receptor Alpha 2 (IL22Ra2)48T/96TY12448Rattus norvegicus (Rat)
肿廇蛋白P63(TP63)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tumor Protein P63 (TP63)48T/96TY12449Homo sapiens (Human)
桥粒芯胶粘蛋白1(DSC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Desmocollin 1 (DSC1)48T/96TY12450Homo sapiens (Human)
夏科雷登氏结晶蛋白(CLC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Charcot Leyden Crystal Protein (CLC)48T/96TY12451Homo sapiens (Human)
防御素α3(DEFα3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Defensin Alpha 3, Neutrophil Specific (DEFa3)48T/96TY12452Homo sapiens (Human)
卵泡抑素样蛋白1(FSTL1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Follistatin Like Protein 1 (FSTL1)48T/96TY12453Homo sapiens (Human)
血小板反应蛋白2(THBS2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Thrombospondin 2 (THBS2)48T/96TY12454Rattus norvegicus (Rat)
晶状体蛋白αB(CRYαB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Crystallin Alpha B (CRYaB)48T/96TY12455Mus musculus (Mouse)
晶状体蛋白αB(CRYαB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Crystallin Alpha B (CRYaB)48T/96TY12456Homo sapiens (Human)
解整合素金属蛋白酶10(ADAM10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for A Disintegrin And Metalloprotease 10 (ADAM10)48T/96TY12457Rattus norvegicus (Rat)
血小板衍生生长因子受体样蛋白(PDGFRL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plaet Derived Growth Factor Receptor Like Protein (PDGFRL)48T/96TY12458Homo sapiens (Human)
血小板衍生生长因子受体样蛋白(PDGFRL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plaet Derived Growth Factor Receptor Like Protein (PDGFRL)48T/96TY12459Mus musculus (Mouse)
饥饿素(GHRL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ghrelin (GHRL)48T/96TY12460Sus scrofa; Porcine (Pig)
血管生成素样蛋白4(ANGPTL4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiopoietin Like Protein 4 (ANGPTL4)48T/96TY12461Mus musculus (Mouse)
着丝粒蛋白F(CENPF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Centromere Protein F (CENPF)48T/96TY12462Homo sapiens (Human)
神经降压素(NT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neurotensin (NT)48T/96TY12463Rattus norvegicus (Rat)
芳香酶(ARO)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aromatase (ARO)48T/96TY12464Rattus norvegicus (Rat)
分泌型卷曲相关蛋白5(SFRP5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secreted Frizzled Related Protein 5 (SFRP5)48T/96TY12465Mus musculus (Mouse)
腺瘤息肉病大肠杆菌蛋白(APC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Adenomatosis Polyposis Coli Protein (APC)48T/96TY12466Homo sapiens (Human)
Sestrin 2蛋白(SESN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sestrin 2 (SESN2)48T/96TY12467Mus musculus (Mouse)
前脑啡肽(PENK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Proenkephalin (PENK)48T/96TY12468Mus musculus (Mouse)
胰岛素样蛋白5(INSL5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Protein 5 (INSL5)48T/96TY12469Homo sapiens (Human)
脯氨酸丰富蛋白4(PRR4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Proline Rich Protein 4, Lacrimal (PRR4)48T/96TY12470Homo sapiens (Human)
Sestrin 3蛋白(SESN3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sestrin 3 (SESN3)48T/96TY12471Mus musculus (Mouse)
Sestrin 1蛋白(SESN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sestrin 1 (SESN1)48T/96TY12472Mus musculus (Mouse)
Jun B原癌基因(JUNB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Jun B Proto Oncogene (JUNB)48T/96TY12473Mus musculus (Mouse)
半胱氨酸蛋白酶抑制剂1(CST1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cystatin 1 (CST1)48T/96TY12474Homo sapiens (Human)
半胱氨酸蛋白酶抑制剂4(CST4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cystatin 4 (CST4)48T/96TY12475Homo sapiens (Human)
博来霉素水解酶(BLMH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bleomycin Hydrolase (BLMH)48T/96TY12476Homo sapiens (Human)
去唾液酸糖蛋白受体2(ASGR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Asialoglycoprotein Receptor 2 (ASGR2)48T/96TY12477Homo sapiens (Human)
镍纹蛋白(METRN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Meteorin (METRN)48T/96TY12478Homo sapiens (Human)
镍纹蛋白(METRN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Meteorin (METRN)48T/96TY12479Mus musculus (Mouse)
Jagged 1蛋白(JAG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Jagged 1 Protein (JAG1)48T/96TY12480Homo sapiens (Human)
溶菌酶(LZM)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Lysozyme (LZM)48T/96TY12481Bos taurus; Bovine (Cattle)
Klotho蛋白(KL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Klotho (KL)48T/96TY12482Homo sapiens (Human)
短蛋白聚糖(BCAN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Brevican (BCAN)48T/96TY12483Homo sapiens (Human)
肝素酶(HPA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heparanase (HPA)48T/96TY12484Mus musculus (Mouse)
各种类型ELISA测定时, 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。