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小鼠神经丝蛋白酶免试剂盒,(NF)ELISA检测试剂盒 ,英文名:NF ELISA Kit ,英文缩写:NF ,常见试剂盒规格:96T/48T,产品别名:小鼠神经丝蛋白ELISA检测试剂盒,NF 检测试剂盒, NF 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠神经丝蛋白酶免试剂盒,(NF)ELISA检测试剂盒 在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
线粒体甘油-3-磷酸酰基转移酶(GPAM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM)48T/96TY12385Rattus norvegicus (Rat)
中间α-球蛋白抑制因子H4(ITIH4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Inter Alpha-Globulin Inhibitor H4 (ITIH4)48T/96TY12386Homo sapiens (Human)
无孢蛋白(ASPN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Asporin (ASPN)48T/96TY12387Homo sapiens (Human)
过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARγC1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a)48T/96TY12388Homo sapiens (Human)
Gremlin 1蛋白(GREM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Gremlin 1 (GREM1)48T/96TY12389Homo sapiens (Human)
游离三碘甲状腺原氨酸(fT3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Free Triiodothyronine (fT3)48T/96TY12390General
骺蛋白聚糖(EPYC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Epiphycan (EPYC)48T/96TY12391Homo sapiens (Human)
生长激素2(GH2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Growth Hormone 2 (GH2)48T/96TY12392Homo sapiens (Human)
次黄嘌呤磷酸核糖基转移酶1(HPRT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)48T/96TY12393Mus musculus (Mouse)
神经纤维网蛋白1(NRP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neuropilin 1 (NRP1)48T/96TY12394Rattus norvegicus (Rat)
线粒体解偶联蛋白2(UCP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Uncoupling Protein 2, Mitochondrial (UCP2)48T/96TY12395Homo sapiens (Human)
保护素(CD59)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protectin (CD59)48T/96TY12396Homo sapiens (Human)
吻素1(KISS1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Kisspeptin 1 (KISS1)48T/96TY12397Rattus norvegicus (Rat)
胰高血糖素样肽2(GLP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucagon Like Peptide 2 (GLP2)48T/96TY12398Homo sapiens (Human)
Ⅰ型胶原α1(COL1α1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Collagen Type I Alpha 1 (COL1a1)48T/96TY12399Homo sapiens (Human)
Ⅺ型胶原α1(COL11α1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Collagen Type XI Alpha 1 (COL11a1)48T/96TY12400Homo sapiens (Human)
透明质酸结合蛋白2(HABP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hyaluronan Binding Protein 2 (HABP2)48T/96TY12401Homo sapiens (Human)
癌胚抗原相关细胞粘附分子7(CEACAM7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Carcinoembryonic Antigen Related Cell Adhesion Molecule 7 (CEACAM7)48T/96TY12402Homo sapiens (Human)
纤维蛋白原相关蛋白1(FGL1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibrinogen Like Protein 1 (FGL1)48T/96TY12403Homo sapiens (Human)
脂解激活脂蛋白受体(LSR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lipolysis Stimulated Lipoprotein Receptor (LSR)48T/96TY12404Homo sapiens (Human)
丝氨酸蛋白酶8(PRSS8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protease, Serine 8 (PRSS8)48T/96TY12405Homo sapiens (Human)
桥粒芯胶粘蛋白1(DSC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Desmocollin 1 (DSC1)48T/96TY12406Mus musculus (Mouse)
G蛋白偶联受体37(GPR37)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for G Protein Coupled Receptor 37 (GPR37)48T/96TY12407Homo sapiens (Human)
转钴胺素蛋白Ⅰ(TCN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transcobalamin I (TCN1)48T/96TY12408Homo sapiens (Human)
分层蛋白(SFN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Stratifin (SFN)48T/96TY12409Homo sapiens (Human)
游离甲状腺素(fT4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Free Thyroxine (fT4)48T/96TY12410General
凝血酶原片段F1+2(F1+2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prothrombin Fragment 1+2 (F1+2)48T/96TY12411Sus scrofa; Porcine (Pig)
轻肽铁蛋白(FTL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ferritin, Light Polypeptide (FTL)48T/96TY12412Homo sapiens (Human)
肌球蛋白ⅤA(MYO5A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myosin VA (MYO5A)48T/96TY12413Mus musculus (Mouse)
氨基乙二酸转氨酶(AADAT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aminoadipate Aminotransferase (AADAT)48T/96TY12414Homo sapiens (Human)
兴奋性氨基酸转运蛋白2(EAAT2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Excitatory Amino Acid Transporter 2 (EAAT2)48T/96TY12415Mus musculus (Mouse)
钠/钾离子转运ATP酶α2肽(ATP1α2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2)48T/96TY12416Mus musculus (Mouse)
钠/钾离子转运ATP酶β1肽(ATP1β1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1)48T/96TY12417Mus musculus (Mouse)
钙离子转运ATP酶B2(ATP2B2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2)48T/96TY12418Mus musculus (Mouse)
泛醌细胞色素C还原酶核心蛋白Ⅱ(UQCRC2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2)48T/96TY12419Mus musculus (Mouse)
RalA结合蛋白1(RALBP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for RalA Binding Protein 1 (RALBP1)48T/96TY12420Homo sapiens (Human)
肌动蛋白相关蛋白2/3复合体亚基4(ARPC4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Actin Related Protein 2/3 Complex Subunit 4 (ARPC4)48T/96TY12421Mus musculus (Mouse)
神经元正五聚蛋白Ⅰ(NPTX1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neuronal Pentraxin I (NPTX1)48T/96TY12422Homo sapiens (Human)
神经元正五聚蛋白Ⅰ(NPTX1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neuronal Pentraxin I (NPTX1)48T/96TY12423Rattus norvegicus (Rat)
尿皮质素2(UCN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Urocortin 2 (UCN2)48T/96TY12424Mus musculus (Mouse)
解整合素金属蛋白酶28(ADAM28)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for A Disintegrin And Metalloprotease 28 (ADAM28)48T/96TY12425Homo sapiens (Human)
尿皮质素3(UCN3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Urocortin 3 (UCN3)48T/96TY12426Mus musculus (Mouse)
半胱氨酸蛋白酶抑制剂B(CSTB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cystatin B (CSTB)48T/96TY12427Homo sapiens (Human)
信号素7A(SEMA7A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Semaphorin 7A (SEMA7A)48T/96TY12428Homo sapiens (Human)
肌肽酶1(CNDP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Carnosine Dipeptidase 1 (CNDP1)48T/96TY12429Homo sapiens (Human)
泛素结合酶E2C(UBE2C)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ubiquitin Conjugating Enzyme E2C (UBE2C)48T/96TY12430Homo sapiens (Human)
Klotho蛋白(KL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Klotho (KL)48T/96TY12431Mus musculus (Mouse)
VEGF共调节趋化因子1(VCC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for VEGF Co Regulated Chemokine 1 (VCC1)48T/96TY12432Homo sapiens (Human)
VGF神经生长因子诱导蛋白(VGF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for VGF Nerve Growth Factor Inducible (VGF)48T/96TY12433Rattus norvegicus (Rat)
细胞因子受体样因子1(CRLF1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytokine Receptor Like Factor 1 (CRLF1)48T/96TY12434Homo sapiens (Human)
各种类型ELISA测定时, 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。