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小鼠Ⅰ型胶原交联羧基末端肽酶免试剂盒,(ⅠCTP)ELISA检测试剂盒 ,英文名:ⅠCTP ELISA Kit ,英文缩写:ⅠCTP ,常见试剂盒规格:96T/48T,产品别名:小鼠Ⅰ型胶原交联羧基末端肽ELISA检测试剂盒,ⅠCTP 检测试剂盒, ⅠCTP 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠Ⅰ型胶原交联羧基末端肽酶免试剂盒,(ⅠCTP)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
穿孔素1(PRF1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Perforin 1 (PRF1)48T/96TY12335Mus musculus (Mouse)
骨膜蛋白(POSTN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Periostin (POSTN)48T/96TY12336Homo sapiens (Human)
脑源性神经营养因子(BDNF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)48T/96TY12337Oryctolagus cuniculus (Rabbit)
雄烯酮(ADT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Androsterone (ADT)48T/96TY12338General
α2-巨球蛋白(α2M)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-2-Macroglobulin (a2M)48T/96TY12339Mus musculus (Mouse)
泛素结合酶E2A(UBE2A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ubiquitin Conjugating Enzyme E2A (UBE2A)48T/96TY12340Mus musculus (Mouse)
Axis抑制蛋白2(AXIN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Axis Inhibition Protein 2 (AXIN2)48T/96TY12341Homo sapiens (Human)
端锚聚合酶1(TNKS1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tankyrase 1 (TNKS1)48T/96TY12342Homo sapiens (Human)
端锚聚合酶2(TNKS2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tankyrase 2 (TNKS2)48T/96TY12343Homo sapiens (Human)
肌纤蛋白(MYOC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myocilin (MYOC)48T/96TY12344Homo sapiens (Human)
肉毒碱棕榈酰基转移酶1A(CPT1A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Carnitine Palmitoyltransferase 1A, Liver (CPT1A)48T/96TY12345Homo sapiens (Human)
叉头框蛋白A2(FOXA2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Forkhead Box Protein A2 (FOXA2)48T/96TY12346Mus musculus (Mouse)
回肠脂肪酸结合蛋白(FABP6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fatty Acid Binding Protein 6, Ileal (FABP6)48T/96TY12347Mus musculus (Mouse)
白细胞关联免疫球蛋白样受体2(LAIR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leukocyte Associated Immunoglobulin Like Receptor 2 (LAIR2)48T/96TY12348Homo sapiens (Human)
核仁素(NCL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nucleolin (NCL)48T/96TY12349Mus musculus (Mouse)
染色框同源物3(CBX3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Chromobox Homolog 3 (CBX3)48T/96TY12350Mus musculus (Mouse)
Cathelicidin抗菌肽(CAMP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cathelicidin Antimicrobial Peptide (CAMP)48T/96TY12351Homo sapiens (Human)
核仁磷酸蛋白(NPM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nucleophosmin (NPM)48T/96TY12352Mus musculus (Mouse)
2',5'-寡腺苷酸合成酶1(OAS1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for 2',5'-Oligoadenylate Synthetase 1 (OAS1)48T/96TY12353Mus musculus (Mouse)
神经纤维网蛋白1(NRP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neuropilin 1 (NRP1)48T/96TY12354Mus musculus (Mouse)
戊糖素(PTD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pentosidine (PTD)48T/96TY12355General
血管抑素(ANG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiostatin (ANG)48T/96TY12356Mus musculus (Mouse)
密封蛋白(OCLN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Occludin (OCLN)48T/96TY12357Homo sapiens (Human)
封闭蛋白1(CLDN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Claudin 1 (CLDN1)48T/96TY12358Homo sapiens (Human)
周期素依赖性激酶抑制因子1A(CDKN1A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cyclin Dependent Kinase Inhibitor 1A (CDKN1A)48T/96TY12359Mus musculus (Mouse)
封闭蛋白4(CLDN4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Claudin 4 (CLDN4)48T/96TY12360Homo sapiens (Human)
成纤维细胞生长因子7(FGF7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor 7 (FGF7)48T/96TY12361Mus musculus (Mouse)
血管活性肠肽受体1(VIPR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vasoactive Intestinal Peptide Receptor 1 (VIPR1)48T/96TY12362Homo sapiens (Human)
失调蛋白1(ATXN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ataxin 1 (ATXN1)48T/96TY12363Homo sapiens (Human)
Ⅵ型胶原α1(COL6α1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Collagen Type VI Alpha 1 (COL6a1)48T/96TY12364Homo sapiens (Human)
中脑星形胶质细胞来源神经营养因子(MANF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)48T/96TY12365Homo sapiens (Human)
酸性葡萄糖苷酶β(GβA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucosidase Beta, Acid (GbA)48T/96TY12366Homo sapiens (Human)
Syncollin蛋白(SYCN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Syncollin (SYCN)48T/96TY12367Homo sapiens (Human)
Thy1细胞表面抗原(Thy1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Thy1 Cell Surface Antigen (Thy1)48T/96TY12368Mus musculus (Mouse)
α1-酸性糖蛋白(α1AGP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)48T/96TY12369Sus scrofa; Porcine (Pig)
P-选择素糖蛋白配体1(PSGL1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for P-Selectin Glycoprotein Ligand 1 (PSGL1)48T/96TY12370Mus musculus (Mouse)
肿瘤坏死因子配体超家族成员7(TNFSF7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 7 (TNFSF7)48T/96TY12371Homo sapiens (Human)
成纤维细胞生长因子受体2(FGFR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor Receptor 2 (FGFR2)48T/96TY12372Homo sapiens (Human)
羧肽酶A2(CPA2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Carboxypeptidase A2, Pancreatic (CPA2)48T/96TY12373Rattus norvegicus (Rat)
羧肽酶A1(CPA1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Carboxypeptidase A1, Pancreatic (CPA1)48T/96TY12374Rattus norvegicus (Rat)
间皮素(MSLN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mesothelin (MSLN)48T/96TY12375Homo sapiens (Human)
V-Ets骨髓成红细胞增多症病毒E26癌基因同源物1(ETS1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1)48T/96TY12376Mus musculus (Mouse)
颗粒体蛋白(GRN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Granulin (GRN)48T/96TY12377Homo sapiens (Human)
分泌型卷曲相关蛋白4(SFRP4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secreted Frizzled Related Protein 4 (SFRP4)48T/96TY12378Mus musculus (Mouse)
视锥蛋白样蛋白1(VSNL1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Visinin Like Protein 1 (VSNL1)48T/96TY12379Homo sapiens (Human)
丝甘蛋白聚糖(SRGN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Serglycin (SRGN)48T/96TY12380Homo sapiens (Human)
Ⅷ型胶原α1(COL8α1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Collagen Type VIII Alpha 1 (COL8a1)48T/96TY12381Homo sapiens (Human)
甲状旁腺激素(PTH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Parathyroid Hormone (PTH)48T/96TY12382Canis familiaris; Canine (Dog)
低氧上调节因子1(HYOU1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hypoxia Up Regulated 1 (HYOU1)48T/96TY12383Homo sapiens (Human)
视黄醇结合蛋白4(RBP4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Retinol Binding Protein 4, Plasma (RBP4)48T/96TY12384Bos taurus; Bovine (Cattle)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。