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小鼠磷脂酰丝氨酸酶免试剂盒,(PS)ELISA检测试剂盒
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更新时间:2016-09-29  |  阅读:1067

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小鼠磷脂酰丝氨酸酶免试剂盒,(PS)ELISA检测试剂盒 ,英文名:PS ELISA Kit ,英文缩写:PS ,常见试剂盒规格:96T/48T,产品别名:小鼠磷脂酰丝氨酸ELISA检测试剂盒,PS 检测试剂盒, PS 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠磷脂酰丝氨酸酶免试剂盒,(PS)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。

激肽释放酶2(KLK2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Kallikrein 2 (KLK2)48T/96TY12235Homo sapiens (Human)
抗神经节苷脂M1抗体(Anti-GM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Anti-Ganglioside M1 Antibody (Anti-GM1)48T/96TY12236Sus scrofa; Porcine (Pig)
血清淀粉样P物质(SAP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Serum Amyloid P Component (SAP)48T/96TY12237Mus musculus (Mouse)
谷胱甘肽S转移酶μ2(GSTμ2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutathione S Transferase Mu 2, Muscle (GSTm2)48T/96TY12238Homo sapiens (Human)
谷胱甘肽S转移酶α4(GSTα4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutathione S Transferase Alpha 4 (GSTa4)48T/96TY12239Homo sapiens (Human)
成肌分化蛋白(MyoD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myogenic Differentiation (MyoD)48T/96TY12240Rattus norvegicus (Rat)
Smad同源物3(Smad3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mothers Against Decapentaplegic Homolog 3 (Smad3)48T/96TY12241Homo sapiens (Human)
膜联蛋白A11(ANXA11)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Annexin A11 (ANXA11)48T/96TY12242Homo sapiens (Human)
膜联蛋白A1(ANXA1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Annexin A1 (ANXA1)48T/96TY12243Homo sapiens (Human)
过氧化还原酶5(PRDX5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Peroxiredoxin 5 (PRDX5)48T/96TY12244Homo sapiens (Human)
氧化低密度脂蛋白(OxLDL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)48T/96TY12245Oryctolagus cuniculus (Rabbit)
桥粒斑蛋白(DSP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Desmoplakin (DSP)48T/96TY12246Homo sapiens (Human)
血管内皮生长因子165(VEGF165)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vascular Endothelial Growth Factor 165 (VEGF165)48T/96TY12247Bos taurus; Bovine (Cattle)
促卵泡素(FSH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Follicle Stimulating Hormone (FSH)48T/96TY12248Mus musculus (Mouse)
胃动蛋白1(GKN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Gastrokine 1 (GKN1)48T/96TY12249Homo sapiens (Human)
α2-巨球蛋白(α2M)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-2-Macroglobulin (a2M)48T/96TY12250Rattus norvegicus (Rat)
纤维胶凝蛋白1(FCN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ficolin 1 (FCN1)48T/96TY12251Homo sapiens (Human)
甘露聚糖结合凝集素关联丝氨酸蛋白酶2(MASP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mannose Associated Serine Protease 2 (MASP2)48T/96TY12252Homo sapiens (Human)
精氨酸(Arg)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Arginine (Arg)48T/96TY12253General
末端补体复合体C5b-9(C5b-9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Terminal Complement Complex C5b-9 (C5b-9)48T/96TY12254Homo sapiens (Human)
肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)48T/96TY12255Canis familiaris; Canine (Dog)
肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)48T/96TY12256Rattus norvegicus (Rat)
簇集蛋白(CLU)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Clusterin (CLU)48T/96TY12257Canis familiaris; Canine (Dog)
胰多肽(PP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pancreatic Polypeptide (PP)48T/96TY12258Mus musculus (Mouse)
穿孔素1(PRF1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Perforin 1 (PRF1)48T/96TY12259Rattus norvegicus (Rat)
α1-抗胰蛋白酶(α1AT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-1-Antitrypsin (a1AT)48T/96TY12260Rattus norvegicus (Rat)
干扰素γ诱导单核因子(MIγ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Monokine Induced By Interferon Gamma (MIg)48T/96TY12261Rattus norvegicus (Rat)
干扰素诱导T-细胞α亚族趋化剂(ITαC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Inducible T-Cell Alpha Chemoattractant (ITaC)48T/96TY12262Rattus norvegicus (Rat)
紧密连接蛋白1(TJP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tight Junction Protein 1 (TJP1)48T/96TY12263Homo sapiens (Human)
诱导型一氧化氮合酶(NOS2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2)48T/96TY12264Canis familiaris; Canine (Dog)
层粘连蛋白(LN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Laminin (LN)48T/96TY12265Homo sapiens (Human)
Endoglin蛋白(ENG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Endoglin (ENG)48T/96TY12266Rattus norvegicus (Rat)
雄激素受体(AR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Androgen Receptor (AR)48T/96TY12267Rattus norvegicus (Rat)
阿黑皮素原(POMC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Proopiomelanocortin (POMC)48T/96TY12268Mus musculus (Mouse)
谷胱甘肽还原酶(GR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutathione Reductase (GR)48T/96TY12269Rattus norvegicus (Rat)
内皮素受体A(ETRA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Endothelin Receptor A (ETRA)48T/96TY12270Homo sapiens (Human)
妊娠相关血浆蛋白A2(PAPPA2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pappalysin 2 (PAPPA2)48T/96TY12271Homo sapiens (Human)
补体成分5a(C5a)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Complement Component 5a (C5a)48T/96TY12272Oryctolagus cuniculus (Rabbit)
乳铁传递蛋白(LTF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactoferrin (LTF)48T/96TY12273Equus caballus; Equine (Horse)
乳铁传递蛋白(LTF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactoferrin (LTF)48T/96TY12274Canis familiaris; Canine (Dog)
乳铁传递蛋白(LTF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactoferrin (LTF)48T/96TY12275Capra hircus; Caprine (Goat)
髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myeloperoxidase (MPO)48T/96TY12276Bos taurus; Bovine (Cattle)
髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myeloperoxidase (MPO)48T/96TY12277Equus caballus; Equine (Horse)
蛋白C抑制因子(PCI)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein C Inhibitor (PCI)48T/96TY12278Homo sapiens (Human)
脯氨酰羧肽酶(PRCP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prolylcarboxypeptidase (PRCP)48T/96TY12279Rattus norvegicus (Rat)
衰老关键蛋白5(FBLN5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibulin 5 (FBLN5)48T/96TY12280Homo sapiens (Human)
纤维蛋白原(FG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibrinogen (FG)48T/96TY12281Bos taurus; Bovine (Cattle)
牙本质涎磷蛋白(DSPP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dentin Sialophosphoprotein (DSPP)48T/96TY12282Homo sapiens (Human)
神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Enolase, Neuron Specific (NSE)48T/96TY12283Oryctolagus cuniculus (Rabbit)
皮质醇结合球蛋白(CBG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Corticosteroid Binding Globulin (CBG)48T/96TY12284Homo sapiens (Human)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。

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