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小鼠小扁豆素结合型甲胎蛋白/甲胎蛋白异质体3酶免试剂盒,(AFP-L3)ELISA检测试剂盒 ,英文名:AFP-L3 ELISA Kit ,英文缩写:AFP-L3 ,常见试剂盒规格:96T/48T,产品别名:小鼠小扁豆素结合型甲胎蛋白/甲胎蛋白异质体3ELISA检测试剂盒,AFP-L3 检测试剂盒, AFP-L3 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠小扁豆素结合型甲胎蛋白/甲胎蛋白异质体3酶免试剂盒,(AFP-L3)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
抗凝血酶(AT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Antithrombin (AT)48T/96TY12035Mus musculus (Mouse)
趋化因子C-C-基元受体2(CCR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Chemokine C-C-Motif Receptor 2 (CCR2)48T/96TY12036Homo sapiens (Human)
雌激素受体β(ERβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Estrogen Receptor Beta (ERb)48T/96TY12037Rattus norvegicus (Rat)
雌激素受体α(ERα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Estrogen Receptor Alpha (ERa)48T/96TY12038Mus musculus (Mouse)
雄激素受体(AR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Androgen Receptor (AR)48T/96TY12039Mus musculus (Mouse)
芳香烃受体(AhR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aryl Hydrocarbon Receptor (AhR)48T/96TY12040Mus musculus (Mouse)
核因子κB受体激活因子(RANκ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B (RANk)48T/96TY12041Homo sapiens (Human)
胃泌素(GT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Gastrin (GT)48T/96TY12042Canis familiaris; Canine (Dog)
极低密度脂蛋白(VLDL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Very Low Density Lipoprotein (VLDL)48T/96TY12043Homo sapiens (Human)
成纤维细胞生长因子4(FGF4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor 4 (FGF4)48T/96TY12044Bos taurus; Bovine (Cattle)
成纤维细胞生长因子4(FGF4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor 4 (FGF4)48T/96TY12045Chicken (Gallus)
成纤维细胞生长因子6(FGF6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor 6 (FGF6)48T/96TY12046Danio rerio (Zebrafish)
成纤维细胞生长因子9(FGF9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor 9 (FGF9)48T/96TY12047Chicken (Gallus)
成纤维细胞生长因子9(FGF9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor 9 (FGF9)48T/96TY12048Sus scrofa; Porcine (Pig)
干扰素α(IFNα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Alpha (IFNa)48T/96TY12049Bos taurus; Bovine (Cattle)
干扰素α(IFNα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Alpha (IFNa)48T/96TY12050Equus caballus; Equine (Horse)
载脂蛋白C1(APOC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein C1 (APOC1)48T/96TY12051Bos taurus; Bovine (Cattle)
氨乙酰丙酸δ脱水酶(ALAD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aminolevulinate Delta Dehydratase (ALAD)48T/96TY12052Homo sapiens (Human)
亚铁螯合酶(FECH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ferrochelatase (FECH)48T/96TY12053Rattus norvegicus (Rat)
70kDa热休克蛋白5(HSPA5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock 70kDa Protein 5 (HSPA5)48T/96TY12054Homo sapiens (Human)
成纤维细胞生长因子3(FGF3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor 3 (FGF3)48T/96TY12055Homo sapiens (Human)
YY肽(PYY)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Peptide YY (PYY)48T/96TY12056Rattus norvegicus (Rat)
Ⅰ类主要组织相容性复合体C(MHCC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Major Histocompatibility Complex Class I C (MHCC)48T/96TY12057Homo sapiens (Human)
胰岛素样生长因子结合蛋白5(IGFBP5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Growth Factor Binding Protein 5 (IGFBP5)48T/96TY12058Bos taurus; Bovine (Cattle)
转甲状腺素蛋白(TTR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transthyretin (TTR)48T/96TY12059Bos taurus; Bovine (Cattle)
突触核蛋白α(SNCα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Synuclein Alpha (SNCa)48T/96TY12060Mus musculus (Mouse)
渗透性糖蛋白(Pgp)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Permeability Glycoprotein (Pgp)48T/96TY12061Mus musculus (Mouse)
Prominin 1蛋白(PROM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prominin 1 (PROM1)48T/96TY12062Homo sapiens (Human)
补体成分5(C5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Complement Component 5 (C5)48T/96TY12063Mus musculus (Mouse)
硬骨素(SOST)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sclerostin (SOST)48T/96TY12064Rattus norvegicus (Rat)
过氧化氢酶(CAT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Catalase (CAT)48T/96TY12065Rattus norvegicus (Rat)
血浆谷胱甘肽过氧化物酶(GPX3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutathione Peroxidase 3, Plasma (GPX3)48T/96TY12066Rattus norvegicus (Rat)
乙酰胆碱酯酶(AchE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Acetylcholinesterase (AchE)48T/96TY12067Rattus norvegicus (Rat)
Ⅹ型胶原(COL10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Collagen Type X (COL10)48T/96TY12068Mus musculus (Mouse)
维生素B1(VB1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vitamin B1 (VB1)48T/96TY12069General
硒蛋白P1(SEPP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Selenoprotein P1, Plasma (SEPP1)48T/96TY12070Mus musculus (Mouse)
硒蛋白P1(SEPP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Selenoprotein P1, Plasma (SEPP1)48T/96TY12071Rattus norvegicus (Rat)
载脂蛋白B(APOB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein B (APOB)48T/96TY12072Homo sapiens (Human)
组织蛋白酶S(CTSS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cathepsin S (CTSS)48T/96TY12073Mus musculus (Mouse)
血红素结合蛋白(HPX)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hemopexin (HPX)48T/96TY12074Bos taurus; Bovine (Cattle)
补体成分4(C4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Complement Component 4 (C4)48T/96TY12075Mus musculus (Mouse)
血管活性肠肽(VIP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vasoactive Intestinal Peptide (VIP)48T/96TY12076Bos taurus; Bovine (Cattle)
内皮抑素(ES)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Endostatin (ES)48T/96TY12077Rattus norvegicus (Rat)
血管抑素(ANG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiostatin (ANG)48T/96TY12078Rattus norvegicus (Rat)
肌肉生长抑制素(MSTN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myostatin (MSTN)48T/96TY12079Rattus norvegicus (Rat)
免疫球蛋白G2(IgG2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Immunoglobulin G2 (IgG2)48T/96TY12080Cavia (Guinea pig )
末端补体复合体C5b-9(C5b-9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Terminal Complement Complex C5b-9 (C5b-9)48T/96T,(AFP-L3)ELISA检测试剂盒Y12081Sus scrofa; Porcine (Pig)
破骨细胞关联受体(OSCAR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Osteoclast Associated Receptor (OSCAR)48T/96TY12082Homo sapiens (Human)
高迁移率族蛋白1(HMG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for High Mobility Group Protein 1 (HMG1)48T/96TY12083Canis familiaris; Canine (Dog)
高迁移率族蛋白1(HMG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for High Mobility Group Protein 1 (HMG1)48T/96TY12084Sus scrofa; Porcine (Pig)
各种类型ELISA测定时,,(AFP-L3)ELISA检测试剂盒一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。