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本产品仅用于科研,不用于临床
小鼠CD19分子酶免试剂盒,(CD19)ELISA检测试剂盒 ,英文名:CD19 ELISA Kit ,英文缩写:CD19 ,常见试剂盒规格:96T/48T,产品别名:小鼠CD19分子ELISA检测试剂盒,CD19 检测试剂盒, CD19 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠CD19分子酶免试剂盒,(CD19)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
基质金属蛋白酶15(MMP15)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 15 (MMP15)48T/96TY11935Homo sapiens (Human)
基质金属蛋白酶15(MMP15)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 15 (MMP15)48T/96TY11936Mus musculus (Mouse)
基质金属蛋白酶15(MMP15)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 15 (MMP15)48T/96TY11937Sus scrofa; Porcine (Pig)
S100钙结合蛋白(S100)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for S100 Calcium Binding Protein (S100)48T/96TY11938Homo sapiens (Human)
角蛋白1(KRT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Keratin 1 (KRT1)48T/96TY11939Homo sapiens (Human)
胰岛素降解酶(IDE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Degrading Enzyme (IDE)48T/96TY11940Homo sapiens (Human)
Lacritin蛋白(LACRT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lacritin (LACRT)48T/96TY11941Homo sapiens (Human)
皮敌菌素(DCD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dermcidin (DCD)48T/96TY11942Homo sapiens (Human)
半乳凝素14(GAL14)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Galectin 14 (GAL14)48T/96TY11943Capra hircus; Caprine (Goat)
半乳凝素14(GAL14)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Galectin 14 (GAL14)48T/96TY11944Homo sapiens (Human)
半乳凝素14(GAL14)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Galectin 14 (GAL14)48T/96TY11945Rhesus monkey (Simian)
肾足蛋白(NPHN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nephrin (NPHN)48T/96TY11946Mus musculus (Mouse)
肌钙蛋白T(TNT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Troponin T (TNT)48T/96TY11947Sus scrofa; Porcine (Pig)
α-胞衬蛋白(SPTAN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-Fodrin (SPTAN1)48T/96TY11948Chicken (Gallus)
谷氨酸脱氢酶(GDH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutamate Dehydrogenase 1 (GDH)48T/96TY11949Bos taurus; Bovine (Cattle)
谷氨酸脱氢酶(GDH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutamate Dehydrogenase 1 (GDH)48T/96TY11950Mus musculus (Mouse)
谷氨酸脱氢酶(GDH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutamate Dehydrogenase 1 (GDH)48T/96TY11951Rattus norvegicus (Rat)
谷胱甘肽过氧化酶1(GPX1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutathione Peroxidase 1 (GPX1)48T/96TY11952Bos taurus; Bovine (Cattle)
谷胱甘肽过氧化酶1(GPX1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutathione Peroxidase 1 (GPX1)48T/96TY11953Sus scrofa; Porcine (Pig)
谷胱甘肽过氧化酶1(GPX1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutathione Peroxidase 1 (GPX1)48T/96TY11954Oryctolagus cuniculus (Rabbit)
乳过氧化物酶(LPO)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactoperoxidase (LPO)48T/96TY11955Bos taurus; Bovine (Cattle)
乳过氧化物酶(LPO)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactoperoxidase (LPO)48T/96TY11956Capra hircus; Caprine (Goat)
弹性蛋白(ELN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Elastin (ELN)48T/96TY11957Rattus norvegicus (Rat)
血管生成素1(ANGPT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiopoietin 1 (ANGPT1)48T/96TY11958Bos taurus; Bovine (Cattle)
血管生成素1(ANGPT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiopoietin 1 (ANGPT1)48T/96TY11959Chicken (Gallus)
胃泌素(GT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Gastrin (GT)48T/96TY11960Rattus norvegicus (Rat)
脑源性神经营养因子(BDNF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)48T/96TY11961Bos taurus; Bovine (Cattle)
脑源性神经营养因子(BDNF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)48T/96TY11962Canis familiaris; Canine (Dog)
脑源性神经营养因子(BDNF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)48T/96TY11963Equus caballus; Equine (Horse)
脑源性神经营养因子(BDNF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)48T/96TY11964Chicken (Gallus)
脑源性神经营养因子(BDNF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)48T/96TY11965Cavia (Guinea pig )
脑源性神经营养因子(BDNF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)48T/96TY11966Sus scrofa; Porcine (Pig)
结缔组织生长因子(CTGF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Connective Tissue Growth Factor (CTGF)48T/96TY11967Bos taurus; Bovine (Cattle)
结缔组织生长因子(CTGF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Connective Tissue Growth Factor (CTGF)48T/96TY11968Capra hircus; Caprine (Goat)
结缔组织生长因子(CTGF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Connective Tissue Growth Factor (CTGF)48T/96TY11969Chicken (Gallus)
结缔组织生长因子(CTGF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Connective Tissue Growth Factor (CTGF)48T/96TY11970Sus scrofa; Porcine (Pig)
结缔组织生长因子(CTGF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Connective Tissue Growth Factor (CTGF)48T/96TY11971Oryctolagus cuniculus (Rabbit)
转铁蛋白受体(TFR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transferrin Receptor (TFR)48T/96TY11972Homo sapiens (Human)
骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)48T/96TY11973Capra hircus; Caprine (Goat)
骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)48T/96TY11974Chicken (Gallus)
骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)48T/96TY11975Sus scrofa; Porcine (Pig)
骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)48T/96TY11976Oryctolagus cuniculus (Rabbit)
骨成型蛋白4(BMP4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 4 (BMP4)48T/96TY11977Bos taurus; Bovine (Cattle)
骨成型蛋白4(BMP4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 4 (BMP4)48T/96TY11978Canis familiaris; Canine (Dog)
骨成型蛋白4(BMP4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 4 (BMP4)48T/96TY11979Capra hircus; Caprine (Goat)
骨成型蛋白4(BMP4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 4 (BMP4)48T/96TY11980Chicken (Gallus)
骨成型蛋白4(BMP4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 4 (BMP4)48T/96TY11981Sus scrofa; Porcine (Pig)
骨成型蛋白4(BMP4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 4 (BMP4)48T/96TY11982Oryctolagus cuniculus (Rabbit)
S100钙结合蛋白(S100)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for S100 Calcium Binding Protein (S100)48T/96TY11983Bos taurus; Bovine (Cattle)
S100钙结合蛋白(S100)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for S100 Calcium Binding Protein (S100)48T/96TY11984Mus musculus (Mouse)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。