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小鼠乳酸脱氢酶酶免试剂盒,(LDH)ELISA检测试剂盒
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更新时间:2016-09-29  |  阅读:1120

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小鼠乳酸脱氢酶酶免试剂盒,(LDH)ELISA检测试剂盒 ,英文名:LDH ELISA Kit ,英文缩写:LDH ,常见试剂盒规格:96T/48T,产品别名:小鼠乳酸脱氢酶ELISA检测试剂盒,LDH 检测试剂盒, LDH 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠乳酸脱氢酶酶免试剂盒,(LDH)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。

转铁蛋白受体2(TFR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transferrin Receptor 2 (TFR2)48T/96TY11785Rattus norvegicus (Rat)
白介素6(IL6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 6 (IL6)48T/96TY11786Bos taurus; Bovine (Cattle)
白介素6(IL6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 6 (IL6)48T/96TY11787Capra hircus; Caprine (Goat)
白介素6(IL6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 6 (IL6)48T/96TY11788Equus caballus; Equine (Horse)
抗脑组织抗体(ABAb)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Anti-Brain Tissue Antibody (ABAb)48T/96TY11789Oryctolagus cuniculus (Rabbit)
白介素8(IL8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 8 (IL8)48T/96TY11790Bos taurus; Bovine (Cattle)
白介素8(IL8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 8 (IL8)48T/96TY11791Capra hircus; Caprine (Goat)
白介素8(IL8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 8 (IL8)48T/96TY11792Equus caballus; Equine (Horse)
白介素9(IL9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 9 (IL9)48T/96TY11793Rhesus monkey (Simian)
抗缪勒管激素(AMH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Anti Mullerian Hormone (AMH)48T/96TY11794Chicken (Gallus)
抗缪勒管激素(AMH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Anti Mullerian Hormone (AMH)48T/96TY11795Mus musculus (Mouse)
瘦素受体(LEPR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leptin Receptor (LEPR)48T/96TY11796Sus scrofa; Porcine (Pig)
白血病抑制因子(LIF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leukemia Inhibitory Factor (LIF)48T/96TY11797Bos taurus; Bovine (Cattle)
白血病抑制因子(LIF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leukemia Inhibitory Factor (LIF)48T/96TY11798Canis familiaris; Canine (Dog)
白血病抑制因子(LIF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leukemia Inhibitory Factor (LIF)48T/96TY11799Capra hircus; Caprine (Goat)
白血病抑制因子(LIF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leukemia Inhibitory Factor (LIF)48T/96TY11800Sus scrofa; Porcine (Pig)
瘦素(LEP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leptin (LEP)48T/96TY11801Canis familiaris; Canine (Dog)
瘦素(LEP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leptin (LEP)48T/96TY11802Capra hircus; Caprine (Goat)
瘦素(LEP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leptin (LEP)48T/96TY11803Equus caballus; Equine (Horse)
L选择素(SELL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Selectin, Leukocyte (SELL)48T/96TY11804Bos taurus; Bovine (Cattle)
单核细胞趋化蛋白2(MCP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Monocyte Chemotactic Protein 2 (MCP2)48T/96TY11805Bos taurus; Bovine (Cattle)
单核细胞趋化蛋白2(MCP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Monocyte Chemotactic Protein 2 (MCP2)48T/96TY11806Canis familiaris; Canine (Dog)
单核细胞趋化蛋白2(MCP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Monocyte Chemotactic Protein 2 (MCP2)48T/96TY11807Equus caballus; Equine (Horse)
单核细胞趋化蛋白2(MCP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Monocyte Chemotactic Protein 2 (MCP2)48T/96TY11808Sus scrofa; Porcine (Pig)
巨噬细胞来源趋化因子(MDC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Derived Chemokine (MDC)48T/96TY11809Bos taurus; Bovine (Cattle)
抗平滑肌抗体(ASMA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Anti-Smooth Muscle Antibody (ASMA)48T/96TY11810Homo sapiens (Human)
组织蛋白酶K(CTSK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cathepsin K (CTSK)48T/96TY11811Rhesus monkey (Simian)
组织蛋白酶K(CTSK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cathepsin K (CTSK)48T/96TY11812Bos taurus; Bovine (Cattle)
组织蛋白酶K(CTSK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cathepsin K (CTSK)48T/96TY11813Canis familiaris; Canine (Dog)
组织蛋白酶K(CTSK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cathepsin K (CTSK)48T/96TY11814Chicken (Gallus)
组织蛋白酶K(CTSK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cathepsin K (CTSK)48T/96TY11815Sus scrofa; Porcine (Pig)
组织蛋白酶K(CTSK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cathepsin K (CTSK)48T/96TY11816Oryctolagus cuniculus (Rabbit)
胰岛素样生长因子结合蛋白6(IGFBP6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6)48T/96TY11817Bos taurus; Bovine (Cattle)
胰岛素样生长因子结合蛋白6(IGFBP6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6)48T/96TY11818Capra hircus; Caprine (Goat)
胰岛素样生长因子结合蛋白6(IGFBP6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6)48T/96TY11819Equus caballus; Equine (Horse)
胰岛素样生长因子结合蛋白6(IGFBP6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6)48T/96TY11820Homo sapiens (Human)
胰岛素样生长因子结合蛋白6(IGFBP6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6)48T/96TY11821Sus scrofa; Porcine (Pig)
抑制素结合蛋白(INHBP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Inhibin Binding Protein (INHBP)48T/96TY11822Homo sapiens (Human)
抑制素结合蛋白(INHBP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Inhibin Binding Protein (INHBP)48T/96TY11823Mus musculus (Mouse)
T-细胞激活连接蛋白(LAT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Linker For Activation Of T-Cell (LAT)48T/96TY11824Rattus norvegicus (Rat)
转运蛋白1(TNPO1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transportin 1 (TNPO1)48T/96TY11825Bos taurus; Bovine (Cattle)
转运蛋白1(TNPO1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transportin 1 (TNPO1)48T/96TY11826Homo sapiens (Human)
巨噬细胞炎性蛋白1α(MIP1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a)48T/96TY11827Bos taurus; Bovine (Cattle)
巨噬细胞炎性蛋白1α(MIP1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a)48T/96TY11828Canis familiaris; Canine (Dog)
尿皮质素1(UCN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Urocortin 1 (UCN1)48T/96TY11829Capra hircus; Caprine (Goat)
尿皮质素1(UCN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Urocortin 1 (UCN1)48T/96TY11830Homo sapiens (Human)
尿皮质素1(UCN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Urocortin 1 (UCN1)48T/96TY11831Mus musculus (Mouse)
尿皮质素1(UCN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Urocortin 1 (UCN1)48T/96TY11832Rattus norvegicus (Rat)
巨噬细胞炎性蛋白1β(MIP1β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY11833Bos taurus; Bovine (Cattle)
巨噬细胞炎性蛋白1β(MIP1β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY11834Canis familiaris; Canine (Dog)
巨噬细胞炎性蛋白1β(MIP1β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY11835Chicken (Gallus)
巨噬细胞炎性蛋白1β(MIP1β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY11836Sus scrofa; Porcine (Pig)
巨噬细胞炎性蛋白1β(MIP1β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY11837Oryctolagus cuniculus (Rabbit)
巨噬细胞炎性蛋白1β(MIP1β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY11838Rhesus monkey (Simian)
巨噬细胞炎性蛋白3α(MIP3α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)48T/96TY11839Bos taurus; Bovine (Cattle)
巨噬细胞炎性蛋白3α(MIP3α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)48T/96TY11840Canis familiaris; Canine (Dog)
巨噬细胞炎性蛋白3α(MIP3α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)48T/96TY11841Sus scrofa; Porcine (Pig)
巨噬细胞炎性蛋白3β(MIP3β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 3 Beta (MIP3b)48T/96TY11842Bos taurus; Bovine (Cattle)
巨噬细胞炎性蛋白3β(MIP3β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 3 Beta (MIP3b)48T/96TY11843Canis familiaris; Canine (Dog)
巨噬细胞炎性蛋白3β(MIP3β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 3 Beta (MIP3b)48T/96TY11844Sus scrofa; Porcine (Pig)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。

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