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小鼠IgM ELISA检测试剂盒
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更新时间:2016-10-16  |  阅读:911

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小鼠IgM ELISA检测试剂盒,英文名:小鼠IgM ELISA试剂盒,英文缩写:小鼠IgM ELISA试剂盒,常见试剂盒规格:96T/48T,产品别名:小鼠IgM ELISA试剂盒小鼠IgMELISA试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠IgM ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。

白介素1α(IL1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY11735Canis familiaris; Canine (Dog)
白介素1α(IL1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY11736Capra hircus; Caprine (Goat)
白介素1α(IL1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY11737Equus caballus; Equine (Horse)
白介素1α(IL1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY11738Cavia (Guinea pig )
白介素1α(IL1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY11739Oryctolagus cuniculus (Rabbit)
防御素β2(DEFβ2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Defensin Beta 2 (DEFb2)48T/96TY11740Capra hircus; Caprine (Goat)
防御素β2(DEFβ2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Defensin Beta 2 (DEFb2)48T/96TY11741Equus caballus; Equine (Horse)
防御素β2(DEFβ2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Defensin Beta 2 (DEFb2)48T/96TY11742Mus musculus (Mouse)
白介素2(IL2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 2 (IL2)48T/96TY11743Equus caballus; Equine (Horse)
免疫球蛋白G1(IgG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Immunoglobulin G1 (IgG1)48T/96TY11744Mus musculus (Mouse)
基质金属蛋白酶12(MMP12)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 12 (MMP12)48T/96TY11745Rattus norvegicus (Rat)
半乳糖苷酶β(GLβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Galactosidase Beta (GLb)48T/96TY11746Bos taurus; Bovine (Cattle)
半乳糖苷酶β(GLβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Galactosidase Beta (GLb)48T/96TY11747Canis familiaris; Canine (Dog)
半乳糖苷酶β(GLβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Galactosidase Beta (GLb)48T/96TY11748Danio rerio (Zebrafish)
半乳糖苷酶β(GLβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Galactosidase Beta (GLb)48T/96TY11749Rhesus monkey (Simian)
前动力蛋白受体1(PKR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prokineticin Receptor 1 (PKR1)48T/96TY11750Bos taurus; Bovine (Cattle)
前动力蛋白受体1(PKR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prokineticin Receptor 1 (PKR1)48T/96TY11751Rattus norvegicus (Rat)
亲环素B(CYPB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cyclophilin B (CYPB)48T/96TY11752Bos taurus; Bovine (Cattle)
亲环素B(CYPB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cyclophilin B (CYPB)48T/96TY11753Chicken (Gallus)
亲环素B(CYPB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cyclophilin B (CYPB)48T/96TY11754Mus musculus (Mouse)
亲环素B(CYPB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cyclophilin B (CYPB)48T/96TY11755Rattus norvegicus (Rat)
亲环素B(CYPB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cyclophilin B (CYPB)48T/96TY11756Oryctolagus cuniculus (Rabbit)
缓激肽(BK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bradykinin (BK)48T/96TY11757Oryctolagus cuniculus (Rabbit)
维生素A(VA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vitamin A (VA)48T/96TY11758General
硫氧化还原蛋白(Trx)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Thioredoxin (Trx)48T/96TY11759Spinacia oleracea (Spinach)
抑肽酶(AP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aprotinin (AP)48T/96TY11760Sus scrofa; Porcine (Pig)
成纤维细胞生长因子10(FGF10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor 10 (FGF10)48T/96TY11761Homo sapiens (Human)
凝血酶原片段F1+2(F1+2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prothrombin Fragment 1+2 (F1+2)48T/96TY11762Mus musculus (Mouse)
Ⅰ型前胶原(PCⅠ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Procollagen I (PCI)48T/96TY11763Mus musculus (Mouse)
WAP四二硫化物核心域蛋白1(WFDC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for WAP Four Disulfide Core Domain Protein 1 (WFDC1)48T/96TY11764Rattus norvegicus (Rat)
补体因子D(CFD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Complement Factor D (CFD)48T/96TY11765Mus musculus (Mouse)
白介素3(IL3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 3 (IL3)48T/96TY11766Canis familiaris; Canine (Dog)
白介素3(IL3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 3 (IL3)48T/96TY11767Capra hircus; Caprine (Goat)
白介素4(IL4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 4 (IL4)48T/96TY11768Capra hircus; Caprine (Goat)
白介素4(IL4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 4 (IL4)48T/96TY11769Equus caballus; Equine (Horse)
白介素5(IL5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 5 (IL5)48T/96TY11770Bos taurus; Bovine (Cattle)
白介素5(IL5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 5 (IL5)48T/96TY11771Canis familiaris; Canine (Dog)
白介素5(IL5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 5 (IL5)48T/96TY11772Capra hircus; Caprine (Goat)
白介素5(IL5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 5 (IL5)48T/96TY11773Equus caballus; Equine (Horse)
白介素5(IL5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 5 (IL5)48T/96TY11774Chicken (Gallus)
膜联蛋白A5(ANXA5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Annexin A5 (ANXA5)48T/96TY11775Bos taurus; Bovine (Cattle)
膜联蛋白A5(ANXA5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Annexin A5 (ANXA5)48T/96TY11776Chicken (Gallus)
膜联蛋白A5(ANXA5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Annexin A5 (ANXA5)48T/96TY11777Rhesus monkey (Simian)
载脂蛋白C1(APOC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein C1 (APOC1)48T/96TY11778Canis familiaris; Canine (Dog)
载脂蛋白C1(APOC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein C1 (APOC1)48T/96TY11779Rattus norvegicus (Rat)
载脂蛋白C1(APOC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein C1 (APOC1)48T/96TY11780Oryctolagus cuniculus (Rabbit)
线粒体肌酸激酶1A(CKMT1A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Creatine Kinase, Mitochondrial 1A (CKMT1A)48T/96TY11781Bos taurus; Bovine (Cattle)
线粒体肌酸激酶1A(CKMT1A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Creatine Kinase, Mitochondrial 1A (CKMT1A)48T/96TY11782Chicken (Gallus)
线粒体肌酸激酶1A(CKMT1A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Creatine Kinase, Mitochondrial 1A (CKMT1A)48T/96TY11783Sus scrofa; Porcine (Pig)
转铁蛋白受体2(TFR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transferrin Receptor 2 (TFR2)48T/96TY11784Mus musculus (Mouse)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。

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