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小鼠IgG ELISA检测试剂盒
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更新时间:2016-10-06  |  阅读:1189

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小鼠IgG ELISA检测试剂盒,英文名:小鼠IgG ELISA试剂盒,英文缩写:小鼠IgG ELISA试剂盒,常见试剂盒规格:96T/48T,产品别名:小鼠IgG ELISA试剂盒小鼠IgGELISA试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠IgG ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。

磷脂酰肌醇蛋白聚糖4(GPC4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glypican 4 (GPC4)48T/96TY11685Mus musculus (Mouse)
干扰素调节因子3(IRF3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Regulatory Factor 3 (IRF3)48T/96TY11686Mus musculus (Mouse)
细胞外基质蛋白1(ECM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Extracellular Matrix Protein 1 (ECM1)48T/96TY11687Homo sapiens (Human)
肾上腺髓质素(ADM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Adrenomedullin (ADM)48T/96TY11688Equus caballus; Equine (Horse)
干扰素β(IFNβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Beta (IFNb)48T/96TY11689Rhesus monkey (Simian)
白介素1受体拮抗剂(IL1RA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY11690Canis familiaris; Canine (Dog)
白介素1受体拮抗剂(IL1RA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY11691Capra hircus; Caprine (Goat)
白介素1受体拮抗剂(IL1RA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY11692Equus caballus; Equine (Horse)
白介素1受体拮抗剂(IL1RA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY11693Oryctolagus cuniculus (Rabbit)
干扰素β(IFNβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Beta (IFNb)48T/96TY11694Equus caballus; Equine (Horse)
基质金属蛋白酶11(MMP11)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 11 (MMP11)48T/96TY11695Rattus norvegicus (Rat)
白介素1受体拮抗剂(IL1RA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY11696Bos taurus; Bovine (Cattle)
白介素18(IL18)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 18 (IL18)48T/96TY11697Capra hircus; Caprine (Goat)
白介素18(IL18)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 18 (IL18)48T/96TY11698Equus caballus; Equine (Horse)
白介素1受体拮抗剂(IL1RA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY11699Rhesus monkey (Simian)
白介素18(IL18)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 18 (IL18)48T/96TY11700Oryctolagus cuniculus (Rabbit)
心钠肽(ANP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Atrial Natriuretic Peptide (ANP)48T/96TY11701Bos taurus; Bovine (Cattle)
心钠肽(ANP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Atrial Natriuretic Peptide (ANP)48T/96TY11702Equus caballus; Equine (Horse)
心钠肽(ANP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Atrial Natriuretic Peptide (ANP)48T/96TY11703Cavia (Guinea pig )
心钠肽(ANP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Atrial Natriuretic Peptide (ANP)48T/96TY11704Sus scrofa; Porcine (Pig)
白介素1受体Ⅱ(IL1R2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Type II (IL1R2)48T/96TY11705Rhesus monkey (Simian)
末端补体复合体C5b-9(C5b-9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Terminal Complement Complex C5b-9 (C5b-9)48T/96TY11706Mus musculus (Mouse)
神经胶质纤维酸性蛋白(GFAP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)48T/96TY11707Bos taurus; Bovine (Cattle)
激活素B(ACVB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Activin B (ACVB)48T/96TY11708Homo sapiens (Human)
Ⅲ型胶原(COL3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Collagen Type III (COL3)48T/96TY11709Bos taurus; Bovine (Cattle)
α2-HS糖蛋白(αHSG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG)48T/96TY11710Bos taurus; Bovine (Cattle)
α2-HS糖蛋白(αHSG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG)48T/96TY11711Capra hircus; Caprine (Goat)
α2-HS糖蛋白(αHSG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG)48T/96TY11712Cavia (Guinea pig )
血小板因子4(PF4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plaet Factor 4 (PF4)48T/96TY11713Bos taurus; Bovine (Cattle)
血小板因子4(PF4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plaet Factor 4 (PF4)48T/96TY11714Capra hircus; Caprine (Goat)
血小板因子4(PF4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plaet Factor 4 (PF4)48T/96TY11715Sus scrofa; Porcine (Pig)
CD99分子(CD99)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 99 (CD99)48T/96TY11716Homo sapiens (Human)
α2-HS糖蛋白(αHSG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG)48T/96TY11717Oryctolagus cuniculus (Rabbit)
α2-HS糖蛋白(αHSG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG)48T/96TY11718Rhesus monkey (Simian)
半乳糖苷酶α(GLα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Galactosidase Alpha (GLa)48T/96TY11719Homo sapiens (Human)
半乳糖苷酶α(GLα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Galactosidase Alpha (GLa)48T/96TY11720Mus musculus (Mouse)
中性粒细胞弹性蛋白酶(ELA2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Elastase 2, Neutrophil (ELA2)48T/96TY11721Canis familiaris; Canine (Dog)
胃蛋白酶原A(PGA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pepsinogen A (PGA)48T/96TY11722Bos taurus; Bovine (Cattle)
胃蛋白酶原A(PGA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pepsinogen A (PGA)48T/96TY11723Mus musculus (Mouse)
胃蛋白酶原C(PGC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pepsinogen C (PGC)48T/96TY11724Chicken (Gallus)
胃蛋白酶原C(PGC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pepsinogen C (PGC)48T/96TY11725Mus musculus (Mouse)
泛素(Ub)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ubiquitin (Ub)48T/96TY11726Bos taurus; Bovine (Cattle)
泛素(Ub)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ubiquitin (Ub)48T/96TY11727Canis familiaris; Canine (Dog)
泛素(Ub)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ubiquitin (Ub)48T/96TY11728Equus caballus; Equine (Horse)
泛素(Ub)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ubiquitin (Ub)48T/96TY11729Chicken (Gallus)
泛素(Ub)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ubiquitin (Ub)48T/96TY11730Cavia (Guinea pig )
泛素(Ub)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ubiquitin (Ub)48T/96TY11731Sus scrofa; Porcine (Pig)
泛素(Ub)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ubiquitin (Ub)48T/96TY11732Oryctolagus cuniculus (Rabbit)
泛素(Ub)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ubiquitin (Ub)48T/96TY11733Rhesus monkey (Simian)
血管生长素(ANG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiogenin (ANG)48T/96TY11734Bos taurus; Bovine (Cattle)
各种类型ELISA测定时, 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。

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