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小鼠瘦素酶免试剂盒,(LEP)ELISA检测试剂盒
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更新时间:2016-10-16  |  阅读:979

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本产品仅用于科研,不用于临床

小鼠瘦素酶免试剂盒,(LEP)ELISA检测试剂盒,英文名:LEP ELISA Kit,英文缩写:LEP ,常见试剂盒规格:96T/48T,产品别名:小鼠瘦素ELISA检测试剂盒,LEP 检测试剂盒,LEP 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠瘦素酶免试剂盒,(LEP)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。

尼克酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4)48T/96TY11285Homo sapiens (Human)
胰岛素样生长因子结合蛋白1(IGFBP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1)48T/96TY11286Sus scrofa; Porcine (Pig)
免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)48T/96TY11287Bos taurus; Bovine (Cattle)
免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)48T/96TY11288Danio rerio (Zebrafish)
反胰岛素样生长因子2(IGF2AS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Growth Factor 2, Antisense (IGF2AS)48T/96TY11289Rattus norvegicus (Rat)
内皮素受体B(ETRB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Endothelin Receptor B (ETRB)48T/96TY11290Homo sapiens (Human)
葡萄糖苷酸酶β(GUSβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucuronidase Beta (GUSb)48T/96TY11291Rattus norvegicus (Rat)
葡萄糖苷酸酶β(GUSβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucuronidase Beta (GUSb)48T/96TY11292Homo sapiens (Human)
葡萄糖苷酸酶β(GUSβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucuronidase Beta (GUSb)48T/96TY11293Mus musculus (Mouse)
β-1,4-半乳糖转移酶1(β4GALT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Beta-1,4-Galactosyltransferase 1 (b4GALT1)48T/96TY11294Rattus norvegicus (Rat)
三叶因子2(TFF2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Trefoil Factor 2 (TFF2)48T/96TY11295Mus musculus (Mouse)
三叶因子2(TFF2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Trefoil Factor 2 (TFF2)48T/96TY11296Rattus norvegicus (Rat)
葡萄糖激酶(GCK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucokinase (GCK)48T/96TY11297Mus musculus (Mouse)
葡萄糖激酶(GCK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucokinase (GCK)48T/96TY11298Rattus norvegicus (Rat)
主要碱性蛋白(MBP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Major Basic Protein (MBP)48T/96TY11299Homo sapiens (Human)
白蛋白(ALB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Albumin (ALB)48T/96TY11300Oryctolagus cuniculus (Rabbit)
血管性血友病因子(vWF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Von Willebrand Factor (vWF)48T/96TY11301Canis familiaris; Canine (Dog)
白介素7(IL7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 7 (IL7)48T/96TY11302Homo sapiens (Human)
红细胞生成素(EPO)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Erythropoietin (EPO)48T/96TY11303Sus scrofa; Porcine (Pig)
血小板内皮细胞粘附分子1(PECAM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plaet/Endothelial Cell Adhesion Molecule (PECAM1)48T/96TY11304Rattus norvegicus (Rat)
平滑肌肌动蛋白α2(ACTα2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Actin Alpha 2, Smooth Muscle (ACTa2)48T/96TY11305Mus musculus (Mouse)
碱性成纤维细胞生长因子(FGF2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)48T/96TY11306Sus scrofa; Porcine (Pig)
轴突生长诱向因子1(Ntn1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Netrin 1 (Ntn1)48T/96TY11307Mus musculus (Mouse)
表面活性物质关联蛋白C(SPC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Surfactant Associated Protein C (SPC)48T/96TY11308Rattus norvegicus (Rat)
Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Toll Like Receptor 2 (TLR2)48T/96TY11309Bos taurus; Bovine (Cattle)
Toll样受体4(TLR4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Toll Like Receptor 4 (TLR4)48T/96TY11310Bos taurus; Bovine (Cattle)
信号传导转录激活因子4(STAT4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Signal Transducer And Activator Of Transcription 4 (STAT4)48T/96TY11311Canis familiaris; Canine (Dog)
血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)48T/96TY11312Mus musculus (Mouse)
血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiotensin I Converting Enzyme 2 (ACE2)48T/96TY11313Homo sapiens (Human)
卵白蛋白(OVA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ovalbumin (OVA)48T/96TY11314General
基质金属蛋白酶16(MMP16)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 16 (MMP16)48T/96TY11315Rattus norvegicus (Rat)
白介素10(IL10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 10 (IL10)48T/96TY11316Chicken (Gallus)
白介素1β(IL1β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Beta (IL1b)48T/96TY11317Canis familiaris; Canine (Dog)
α-血红蛋白稳定蛋白(αHSP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP)48T/96TY11318Rattus norvegicus (Rat)
纽蛋白(VCL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vinculin (VCL)48T/96TY11319Mus musculus (Mouse)
葡萄糖转运蛋白4(GLUT4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucose Transporter 4 (GLUT4)48T/96TY11320Rattus norvegicus (Rat)
癌胚抗原(CEA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Carcinoembryonic Antigen (CEA)48T/96TY11321Rattus norvegicus (Rat)
尼克酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4)48T/96TY11322Mus musculus (Mouse)
血管性血友病因子裂解蛋白酶(vWFcp)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Von Willebrand Factor Cleaving Protease (vWFCP)48T/96TY11323Homo sapiens (Human)
催乳素(PRL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prolactin (PRL)48T/96TY11324Sus scrofa; Porcine (Pig)
神经调节肽U受体1(NMUR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neuromedin U Receptor 1 (NMUR1)48T/96TY11325Homo sapiens (Human)
八聚体结合转录因子4(OCT4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Octamer Binding Transcription Factor 4 (OCT4)48T/96TY11326Mus musculus (Mouse)
白介素4(IL4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 4 (IL4)48T/96TY11327Bos taurus; Bovine (Cattle)
基质细胞衍生因子2(SDF2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Stromal Cell Derived Factor 2 (SDF2)48T/96TY11328Rattus norvegicus (Rat)
乳过氧化物酶(LPO)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactoperoxidase (LPO)48T/96TY11329Rattus norvegicus (Rat)
乳过氧化物酶(LPO)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lactoperoxidase (LPO)48T/96TY11330Mus musculus (Mouse)
γ-谷氨酰转移酶1(γGT1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Gamma-Glutamyltransferase 1 (gGT1)48T/96TY11331Mus musculus (Mouse)
基质金属蛋白酶13(MMP13)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 13 (MMP13)48T/96TY11332Oryctolagus cuniculus (Rabbit)
蛋白C(PROC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein C (PROC)48T/96TY11333Sus scrofa; Porcine (Pig)
肝素结合性表皮生长因子(HBEGF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF)48T/96TY11334Mus musculus (Mouse)
各种类型ELISA测定时, 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。

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