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小鼠白介素10酶免试剂盒,(IL-10)ELISA检测试剂盒
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更新时间:2016-09-29  |  阅读:1014

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小鼠白介素10酶免试剂盒,(IL-10)ELISA检测试剂盒 ,英文名:IL-10 ELISA Kit ,英文缩写:IL-10 ,常见试剂盒规格:96T/48T,产品别名:小鼠白介素10ELISA检测试剂盒,IL-10 检测试剂盒, IL-10 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠白介素10酶免试剂盒,(IL-10)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。嗅素4(OLFM4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Olfactomedin 4 (OLFM4)48T/96TY10765Bos taurus; Bovine (Cattle)
抑瘤素M(OSM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Oncostatin M (OSM)48T/96TY10766Rattus norvegicus (Rat)
肌肌酸激酶(CKM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Creatine Kinase, Muscle (CKM)48T/96TY10767Rattus norvegicus (Rat)
骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Osteoprotegerin (OPG)48T/96TY10768Rattus norvegicus (Rat)
胆囊收缩素A受体(CCKAR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cholecystokinin A Receptor (CCKAR)48T/96TY10769Rattus norvegicus (Rat)
基质金属蛋白酶8(MMP8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 8 (MMP8)48T/96TY10770Rattus norvegicus (Rat)
基质金属蛋白酶8(MMP8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 8 (MMP8)48T/96TY10771Mus musculus (Mouse)
基质金属蛋白酶7(MMP7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 7 (MMP7)48T/96TY10772Rattus norvegicus (Rat)
基质金属蛋白酶7(MMP7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 7 (MMP7)48T/96TY10773Mus musculus (Mouse)
基质金属蛋白酶3(MMP3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 3 (MMP3)48T/96TY10774Rattus norvegicus (Rat)
基质金属蛋白酶2(MMP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 2 (MMP2)48T/96TY10775Rattus norvegicus (Rat)
基质金属蛋白酶13(MMP13)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 13 (MMP13)48T/96TY10776Rattus norvegicus (Rat)
基质金属蛋白酶13(MMP13)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 13 (MMP13)48T/96TY10777Mus musculus (Mouse)
基质金属蛋白酶10(MMP10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 10 (MMP10)48T/96TY10778Rattus norvegicus (Rat)
基质金属蛋白酶10(MMP10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 10 (MMP10)48T/96TY10779Mus musculus (Mouse)
基质金属蛋白酶1(MMP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 1 (MMP1)48T/96TY10780Rattus norvegicus (Rat)
基质金属蛋白酶1(MMP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 1 (MMP1)48T/96TY10781Mus musculus (Mouse)
巨噬细胞炎性蛋白3β(MIP3β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 3 Beta (MIP3b)48T/96TY10782Rattus norvegicus (Rat)
胱天蛋白酶11(CASP11)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Caspase 11 (CASP11)48T/96TY10783Rattus norvegicus (Rat)
胱天蛋白酶11(CASP11)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Caspase 11 (CASP11)48T/96TY10784Homo sapiens (Human)
巨噬细胞炎性蛋白1β(MIP1β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY10785Rattus norvegicus (Rat)
巨噬细胞炎性蛋白1α(MIP1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a)48T/96TY10786Rattus norvegicus (Rat)
巨噬细胞来源趋化因子(MDC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Derived Chemokine (MDC)48T/96TY10787Rattus norvegicus (Rat)
巨噬细胞集落刺激因子(MCSF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Colony Stimulating Factor 1, Macrophage (MCSF)48T/96TY10788Rattus norvegicus (Rat)
单核细胞趋化蛋白3(MCP3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Monocyte Chemotactic Protein 3 (MCP3)48T/96TY10789Rattus norvegicus (Rat)
单核细胞趋化蛋白3(MCP3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Monocyte Chemotactic Protein 3 (MCP3)48T/96TY10790Mus musculus (Mouse)
单核细胞趋化蛋白2(MCP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Monocyte Chemotactic Protein 2 (MCP2)48T/96TY10791Mus musculus (Mouse)
单核细胞趋化蛋白1(MCP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)48T/96TY10792Rattus norvegicus (Rat)
单核细胞趋化蛋白1(MCP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)48T/96TY10793Mus musculus (Mouse)
L选择素(SELL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Selectin, Leukocyte (SELL)48T/96TY10794Rattus norvegicus (Rat)
白血病抑制因子(LIF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leukemia Inhibitory Factor (LIF)48T/96TY10795Rattus norvegicus (Rat)
瘦素(LEP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leptin (LEP)48T/96TY10796Rattus norvegicus (Rat)
瘦素受体(LEPR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leptin Receptor (LEPR)48T/96TY10797Rattus norvegicus (Rat)
白介素5(IL5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 5 (IL5)48T/96TY10798Rattus norvegicus (Rat)
白介素3(IL3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 3 (IL3)48T/96TY10799Rattus norvegicus (Rat)
N-乙酰β-D-氨基葡萄糖苷酶(NAGase)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for N-Acetyl Beta-D-Glucosaminidase (NAGase)48T/96TY10800Rattus norvegicus (Rat)
N-乙酰β-D-氨基葡萄糖苷酶(NAGase)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for N-Acetyl Beta-D-Glucosaminidase (NAGase)48T/96TY10801Mus musculus (Mouse)
白介素1受体Ⅰ(IL1R1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Type I (IL1R1)48T/96TY10802Rattus norvegicus (Rat)
白介素1受体Ⅰ(IL1R1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Type I (IL1R1)48T/96TY10803Mus musculus (Mouse)
白介素1受体Ⅱ(IL1R2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Type II (IL1R2)48T/96TY10804Rattus norvegicus (Rat)
白介素1受体Ⅱ(IL1R2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Receptor Type II (IL1R2)48T/96TY10805Mus musculus (Mouse)
白介素18(IL18)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 18 (IL18)48T/96TY10806Homo sapiens (Human)
白介素17(IL17)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 17 (IL17)48T/96TY10807Rattus norvegicus (Rat)
白介素16(IL16)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 16 (IL16)48T/96TY10808Rattus norvegicus (Rat)
白介素15(IL15)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 15 (IL15)48T/96TY10809Rattus norvegicus (Rat)
白介素13(IL13)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 13 (IL13)48T/96TY10810Rattus norvegicus (Rat)
乙酰辅酶A羧化酶α(ACACα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Acetyl Coenzyme A Carboxylase Alpha (ACACa)48T/96TY10811Rattus norvegicus (Rat)
促生长激素释放激素(GHRH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Growth Hormone Releasing Hormone (GHRH)48T/96TY10812Homo sapiens (Human)
促生长激素释放激素(GHRH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Growth Hormone Releasing Hormone (GHRH)48T/96TY10813Mus musculus (Mouse)
甲状旁腺激素(PTH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Parathyroid Hormone (PTH)48T/96TY10814Rattus norvegicus (Rat)
甲状旁腺激素(PTH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Parathyroid Hormone (PTH)48T/96TY10815Mus musculus (Mouse)
前列腺素E合酶2(PTGES2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prostaglandin E Synthase 2 (PTGES2)48T/96TY10816Mus musculus (Mouse)
去甲肾上腺素(NE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Noradrenaline (NE)48T/96TY10817General
结缔组织生长因子(CTGF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Connective Tissue Growth Factor (CTGF)48T/96TY10818Homo sapiens (Human)
结缔组织生长因子(CTGF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Connective Tissue Growth Factor (CTGF)48T/96TY10819Rattus norvegicus (Rat)
结缔组织生长因子(CTGF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Connective Tissue Growth Factor (CTGF)48T/96TY10820Mus musculus (Mouse)
维生素D5(VD5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vitamin D5 (VD5)48T/96TY10821Homo sapiens (Human)
组织因子(TF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tissue Factor (TF)48T/96TY10822Homo sapiens (Human)
组织因子(TF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tissue Factor (TF)48T/96TY10823Rattus norvegicus (Rat)
组织因子(TF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tissue Factor (TF)48T/96TY10824Mus musculus (Mouse)
组织型纤溶酶原激活因子(tPA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plasminogen Activator, Tissue (tPA)48T/96TY10825Homo sapiens (Human)
各种类型ELISA测定时,  一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。

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