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小鼠趋化因子酶免试剂盒,(fractalkine/CX3CL1) ELISA检测试剂盒,英文名:fractalkine/CX3CL1) ELISA试剂盒,英文缩写:fractalkine/CX3CL1) ELISA试剂盒,常见试剂盒规格:96T/48T,产品别名:小鼠趋化因子ELISA检测试剂盒,fractalkine/CX3CL1 检测试剂盒,fractalkine/CX3CL1 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠趋化因子酶免试剂盒,(fractalkine/CX3CL1) ELISA检测试剂盒 在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
尼克酰胺腺嘌呤二核苷酸磷酸氧化酶5(NOX5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5 (NOX5)48T/96TY10585Oryctolagus cuniculus (Rabbit)
基质金属蛋白酶9(MMP9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 9 (MMP9)48T/96TY10586Oryctolagus cuniculus (Rabbit)
甲状旁腺激素(PTH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Parathyroid Hormone (PTH)48T/96TY10587Homo sapiens (Human)
组织金属蛋白酶抑制因子1(TIMP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)48T/96TY10588Oryctolagus cuniculus (Rabbit)
脂蛋白关联磷脂酶A2(LpPLA2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipase A2, Lipoprotein Associated (LpPLA2)48T/96TY10589Canis familiaris; Canine (Dog)
碱性成纤维细胞生长因子(FGF2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)48T/96TY10590Oryctolagus cuniculus (Rabbit)
巨噬细胞炎性蛋白相关蛋白1(MRP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Inflammatory Protein Related Protein 1 (MRP1)48T/96TY10591Rattus norvegicus (Rat)
内皮型一氧化氮合酶(NOS3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3)48T/96TY10592Canis familiaris; Canine (Dog)
甲状腺素受体β(THRβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Thyroid Hormone Receptor Beta (THRb)48T/96TY10593Homo sapiens (Human)
血管细胞粘附分子1(VCAM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1)48T/96TY10594Oryctolagus cuniculus (Rabbit)
热休克蛋白β2(HSPβ2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock Protein Beta 2 (HSPb2)48T/96TY10595Chicken (Gallus)
免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Immunoglobulin G (IgG)48T/96TY10596Oryctolagus cuniculus (Rabbit)
免疫球蛋白M(IgM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Immunoglobulin M (IgM)48T/96TY10597Oryctolagus cuniculus (Rabbit)
内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Endothelin 1 (EDN1)48T/96TY10598Oryctolagus cuniculus (Rabbit)
缓激肽(BK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bradykinin (BK)48T/96TY10599Mus musculus (Mouse)
髓鞘碱性蛋白(MBP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myelin Basic Protein (MBP)48T/96TY10600Oryctolagus cuniculus (Rabbit)
神经营养因子4(NT4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neurotrophin 4 (NT4)48T/96TY10601Xenopus (Clawed frog)
瘦素(LEP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leptin (LEP)48T/96TY10602Oryctolagus cuniculus (Rabbit)
抗甲状腺微粒体抗体(ATMA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Anti-Thyroid Microsome Antibody (ATMA)48T/96TY10603Homo sapiens (Human)
细胞间粘附分子1(ICAM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1)48T/96TY10604Oryctolagus cuniculus (Rabbit)
细胞间粘附分子2(ICAM2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Intercellular Adhesion Molecule 2 (ICAM2)48T/96TY10605Oryctolagus cuniculus (Rabbit)
纤溶酶原激活物抑制因子1(PAI1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plasminogen Activator Inhibitor 1 (PAI1)48T/96TY10606Oryctolagus cuniculus (Rabbit)
内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Endothelin 1 (EDN1)48T/96TY10607Rattus norvegicus (Rat)
纤溶酶原激活物抑制因子2(PAI2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plasminogen Activator Inhibitor 2 (PAI2)48T/96TY10608Oryctolagus cuniculus (Rabbit)
蛋白C(PROC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein C (PROC)48T/96TY10609Oryctolagus cuniculus (Rabbit)
血栓调节蛋白(TM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Thrombomodulin (TM)48T/96TY10610Oryctolagus cuniculus (Rabbit)
过氧化物酶体增殖物激活受体γ(PPARγ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Peroxisome Proliferator Activated Receptor Gamma (PPARg)48T/96TY10611Oryctolagus cuniculus (Rabbit)
血小板衍生生长因子A(PDGFA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plaet Derived Growth Factor Subunit A (PDGFA)48T/96TY10612Oryctolagus cuniculus (Rabbit)
补体成分4a(C4a)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Complement Component 4a (C4a)48T/96TY10613Oryctolagus cuniculus (Rabbit)
胰岛素(INS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin (INS)48T/96TY10614Oryctolagus cuniculus (Rabbit)
胰岛素样生长因子1(IGF1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Growth Factor 1 (IGF1)48T/96TY10615Oryctolagus cuniculus (Rabbit)
胰岛素样生长因子2(IGF2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Growth Factor 2 (IGF2)48T/96TY10616Oryctolagus cuniculus (Rabbit)
肾素(REN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Renin (REN)48T/96TY10617Oryctolagus cuniculus (Rabbit)
组织因子(TF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tissue Factor (TF)48T/96TY10618Oryctolagus cuniculus (Rabbit)
内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Endothelin 1 (EDN1)48T/96TY10619Homo sapiens (Human)
半胱氨酸蛋白酶抑制剂3(CST3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cystatin 3 (CST3)48T/96TY10620Oryctolagus cuniculus (Rabbit)
骨桥素(OPN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Osteopontin (OPN)48T/96TY10621Canis familiaris; Canine (Dog)
前列腺素E1(PGE1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prostaglandin E1 (PGE1)48T/96TY10622General
前列腺素E合酶2(PTGES2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prostaglandin E Synthase 2 (PTGES2)48T/96TY10623Homo sapiens (Human)
前列腺素E合酶2(PTGES2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prostaglandin E Synthase 2 (PTGES2)48T/96TY10624Rattus norvegicus (Rat)
铜蓝蛋白(CP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ceruloplasmin (CP)48T/96TY10625Mus musculus (Mouse)
醛固酮(ALD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aldosterone (ALD)48T/96TY10626General
血小板因子4(PF4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plaet Factor 4 (PF4)48T/96TY10627Homo sapiens (Human)
热休克转录因子4(HSF4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock Transcription Factor 4 (HSF4)48T/96TY10628Mus musculus (Mouse)
血小板因子4(PF4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plaet Factor 4 (PF4)48T/96TY10629Mus musculus (Mouse)
血小板因子4(PF4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plaet Factor 4 (PF4)48T/96TY10630Rattus norvegicus (Rat)
维生素K1(VK1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vitamin K1 (VK1)48T/96TY10631General
C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for C Reactive Protein (CRP)48T/96TY10632Sus scrofa; Porcine (Pig)
神经肽Y(NPY)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neuropeptide Y (NPY)48T/96TY10633Homo sapiens (Human)
神经肽Y(NPY)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neuropeptide Y (NPY)48T/96TY10634Rattus norvegicus (Rat)
神经肽Y(NPY)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neuropeptide Y (NPY)48T/96TY10635Mus musculus (Mouse)
神经肽Y(NPY)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neuropeptide Y (NPY)48T/96TY10636Bos taurus; Bovine (Cattle)
白介素12A(IL12A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 12A (IL12A)48T/96TY10637Chicken (Gallus)
载脂蛋白B(APOB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein B (APOB)48T/96TY10638Sus scrofa; Porcine (Pig)
细胞绒毛蛋白(CVL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytovillin (CVL)48T/96TY10639Rattus norvegicus (Rat)
大型中性氨基酸转运蛋白1(LAT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Large Neutral Amino Acid Transporter 1 (LAT1)48T/96TY10640Homo sapiens (Human)
白介素8受体β(IL8Rβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 8 Receptor Beta (IL8Rb)48T/96TY10641Rattus norvegicus (Rat)
白介素34(IL34)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 34 (IL34)48T/96TY10642Homo sapiens (Human)
白介素34(IL34)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 34 (IL34)48T/96TY10643Mus musculus (Mouse)
强啡肽(Dyn)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Big Dynorphin (Dyn)48T/96TY10644Mus musculus (Mouse)
脑啡肽(ENK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Enkephalin (ENK)48T/96TY10645Mus musculus (Mouse)
成纤维生长因子受体底物2(FRS2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor Receptor Substrate 2 (FRS2)48T/96TY10646Rattus norvegicus (Rat)
白细胞介素24(IL24)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 24 (IL24)48T/96TY10647Homo sapiens (Human)
金属硫蛋白1(MT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Metallothionein 1 (MT1)48T/96TY10648Mus musculus (Mouse)
肌肉生长抑制素(MSTN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myostatin (MSTN)48T/96TY10649Mus musculus (Mouse)
碳酸酐酶Ⅱ(CA2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Carbonic Anhydrase II (CA2)48T/96TY10650Chicken (Gallus)
雨蛙素(INN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ceruletide (INN)48T/96TY10651General
α2-HS糖蛋白(αHSG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG)48T/96TY10652Mus musculus (Mouse)
核因子κB受体激活因子配基(RANκL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)48T/96TY10653Chicken (Gallus)
早期生长应答因子4(EGR4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Early Growth Response Protein 4 (EGR4)48T/96TY10654Mus musculus (Mouse)
葡萄糖6磷酸脱氢酶(G6PD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucose 6 Phosphate Dehydrogenase (G6PD)48T/96TY10655Homo sapiens (Human)
葡萄糖6磷酸脱氢酶(G6PD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucose 6 Phosphate Dehydrogenase (G6PD)48T/96TY10656Rattus norvegicus (Rat)
谷氨酸脱羧酶2(GAD2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutamate Decarboxylase 2, Acid (GAD2)48T/96TY10657Rattus norvegicus (Rat)
谷氨酸脱羧酶2(GAD2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutamate Decarboxylase 2, Acid (GAD2)48T/96TY10658Mus musculus (Mouse)
山梨醇脱氢酶(SDH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sorbitol Dehydrogenase (SDH)48T/96TY10659Rattus norvegicus (Rat)
硫氧还蛋白结合蛋白2(TBP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Thioredoxin Binding Protein 2 (TBP2)48T/96TY10660Mus musculus (Mouse)
细胞色素P450家族成员7A1(CYP7A1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytochrome P450 7A1 (CYP7A1)48T/96TY10661Homo sapiens (Human)
血红素结合蛋白(HPX)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hemopexin (HPX)48T/96TY10662Rattus norvegicus (Rat)
谷丙转氨酶(ALT)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Alanine Aminotransferase (ALT)48T/96TY10663Cavia (Guinea pig )
谷丙转氨酶(ALT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alanine Aminotransferase (ALT)48T/96TY10664Homo sapiens (Human)
谷丙转氨酶(ALT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alanine Aminotransferase (ALT)48T/96TY10665Sus scrofa; Porcine (Pig)
各种类型ELISA测定时, 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。