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人抗乙型肝炎病毒表面抗体酶免试剂盒,(HBsAb)ELISA检测试剂盒 ,英文名:HBsAb ELISA Kit ,英文缩写:HBsAb ,常见试剂盒规格:96T/48T,产品别名:人抗乙型肝炎病毒表面抗体ELISA检测试剂盒,HBsAb 检测试剂盒, HBsAb 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人抗乙型肝炎病毒表面抗体酶免试剂盒,(HBsAb)ELISA检测试剂盒 在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重
激肽释放酶6(KLK6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Kallikrein 6 (KLK6)48T/96TY09185Homo sapiens (Human)
激肽释放酶8(KLK8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Kallikrein 8 (KLK8)48T/96TY09186Homo sapiens (Human)
晚期糖基化终末产物受体(AGER)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Advanced Glycosylation End Product Specific Receptor (AGER)48T/96TY09187Homo sapiens (Human)
ATP结合盒转运蛋白G1(ABCG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for ATP Binding Cassette Transporter G1 (ABCG1)48T/96TY09188Homo sapiens (Human)
调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)48T/96TY09189Mus musculus (Mouse)
胸腺激活调节趋化因子(TARC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Thymus Activation Regulated Chemokine (TARC)48T/96TY09190Homo sapiens (Human)
蛋白激酶D1(PRKD1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Kinase D1 (PKD1)48T/96TY09191Rattus norvegicus (Rat)
蛋白激酶D1(PRKD1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Kinase D1 (PKD1)48T/96TY09192Mus musculus (Mouse)
V-Myc骨髓细胞瘤病毒癌基因同源物(MYC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)48T/96TY09193Homo sapiens (Human)
V-Fos-FBJ骨肉瘤病毒癌基因同源物(FOS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS)48T/96TY09194Homo sapiens (Human)
V-Jun肉瘤病毒癌基因同源物(JUN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN)48T/96TY09195Homo sapiens (Human)
血小板衍生生长因子B(PDGFB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plaet Derived Growth Factor Subunit B (PDGFB)48T/96TY09196Homo sapiens (Human)
V-Ha-Ras肉瘤病毒癌基因同源物(HRAS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS)48T/96TY09197Homo sapiens (Human)
CD320分子(CD320)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 320 (CD320)48T/96TY09198Rattus norvegicus (Rat)
CD320分子(CD320)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 320 (CD320)48T/96TY09199Mus musculus (Mouse)
基质金属蛋白酶3(MMP3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 3 (MMP3)48T/96TY09200Oryctolagus cuniculus (Rabbit)
环加氧酶1(PTGS1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prostaglandin Endoperoxide Synthase 1 (PTGS1)48T/96TY09201Bos taurus; Bovine (Cattle)
环加氧酶1(PTGS1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prostaglandin Endoperoxide Synthase 1 (PTGS1)48T/96TY09202Homo sapiens (Human)
环加氧酶1(PTGS1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prostaglandin Endoperoxide Synthase 1 (PTGS1)48T/96TY09203Rattus norvegicus (Rat)
环加氧酶1(PTGS1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prostaglandin Endoperoxide Synthase 1 (PTGS1)48T/96TY09204Mus musculus (Mouse)
骨形成蛋白6(BMP6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 6 (BMP6)48T/96TY09205Homo sapiens (Human)
骨形成蛋白6(BMP6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 6 (BMP6)48T/96TY09206Rattus norvegicus (Rat)
骨形成蛋白6(BMP6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Morphogenetic Protein 6 (BMP6)48T/96TY09207Mus musculus (Mouse)
亲环素A(CYPA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cyclophilin A (CYPA)48T/96TY09208Mus musculus (Mouse)
血小板因子4(PF4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plaet Factor 4 (PF4)48T/96TY09209Oryctolagus cuniculus (Rabbit)
透明质酸结合蛋白1(HABP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hyaluronan Binding Protein 1 (HABP1)48T/96TY09210Homo sapiens (Human)
透明质酸结合蛋白1(HABP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hyaluronan Binding Protein 1 (HABP1)48T/96TY09211Rattus norvegicus (Rat)
透明质酸结合蛋白1(HABP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hyaluronan Binding Protein 1 (HABP1)48T/96TY09212Mus musculus (Mouse)
钙结合蛋白(CR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calretinin (CR)48T/96TY09213Homo sapiens (Human)
钙结合蛋白(CR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calretinin (CR)48T/96TY09214Rattus norvegicus (Rat)
钙结合蛋白(CR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calretinin (CR)48T/96TY09215Mus musculus (Mouse)
155kDa核孔蛋白(NUP155)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nucleoporin 155kDa (NUP155)48T/96TY09216Homo sapiens (Human)
肾上腺素能受体α1A(ADRα1A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Adrenergic Receptor Alpha 1A (ADRa1A)48T/96TY09217Homo sapiens (Human)
多巴胺受体D1(DRD1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dopamine Receptor D1 (DRD1)48T/96TY09218Homo sapiens (Human)
CD28分子(CD28)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 28 (CD28)48T/96TY09219Homo sapiens (Human)
不对称二甲基精氨酸(ADMA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Asymmetrical Dimethylarginine (ADMA)48T/96TY09220General
野鼠色基因相关蛋白(AGRP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Agouti Related Protein (AGRP)48T/96TY09221Homo sapiens (Human)
集聚蛋白(AGRN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Agrin (AGRN)48T/96TY09222Homo sapiens (Human)
补体C5转化酶(C5c)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Complement C5 Convertase (C5c)48T/96TY09223Homo sapiens (Human)
补体成分4b(C4b)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Complement Component 4b (C4b)48T/96TY09224Homo sapiens (Human)
补体成分8β(C8β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Complement Component 8b (C8b)48T/96TY09225Homo sapiens (Human)
多效生长因子(PTN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pleiotrophin (PTN)48T/96TY09226Homo sapiens (Human)
弹性蛋白酶4(ELA4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Elastase 4 (ELA4)48T/96TY09227Homo sapiens (Human)
阿黑皮素原(POMC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Proopiomelanocortin (POMC)48T/96TY09228Homo sapiens (Human)
分泌性白细胞蛋白酶抑制因子(SLPI)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI)48T/96TY09229Homo sapiens (Human)
谷胱甘肽还原酶(GR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutathione Reductase (GR)48T/96TY09230Homo sapiens (Human)
前胶原C端蛋白酶增强子1(PCPE1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Procollagen C Proteinase Enhancer 1 (PCPE1)48T/96TY09231Homo sapiens (Human)
穿孔素1(PRF1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Perforin 1 (PRF1)48T/96T 人抗乙型肝炎病毒表面抗体酶免试剂盒,(HBsAb)ELISA检测试剂盒 Y09232Homo sapiens (Human)
cAMP应答元件结合蛋白(CREB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cyclic AMP Response Element Binding Protein (CREB)48T/96TY09233Homo sapiens (Human)
微管关联蛋白4(MAP4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Microtubule Associated Protein 4 (MAP4)48T/96TY09234Homo sapiens (Human)
各种类型ELISA测定时,人抗乙型肝炎病毒表面抗体酶免试剂盒,(HBsAb)ELISA检测试剂盒 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。