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人抗乙型肝炎病毒核心抗体酶免试剂盒,(HBcAb)ELISA检测试剂盒
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更新时间:2016-10-16  |  阅读:1246

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人抗乙型肝炎病毒核心抗体酶免试剂盒,(HBcAb)ELISA检测试剂盒 ,英文名:HBcAb ELISA Kit ,英文缩写:HBcAb ,常见试剂盒规格:96T/48T,产品别名:人抗乙型肝炎病毒核心抗体ELISA检测试剂盒,HBcAb 检测试剂盒, HBcAb 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人抗乙型肝炎病毒核心抗体酶免试剂盒,(HBcAb)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重

 

纤维蛋白原α(FGα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibrinogen Alpha (FGa)48T/96TY09085Homo sapiens (Human)
丝裂原激活蛋白激酶8(MAPK8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mitogen Activated Protein Kinase 8 (MAPK8)48T/96TY09086Homo sapiens (Human)
155kDa核孔蛋白(NUP155)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nucleoporin 155kDa (NUP155)48T/96TY09087Mus musculus (Mouse)
四旋蛋白27(TSPAN27)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tetraspanin 27 (TSPAN27)48T/96TY09088Homo sapiens (Human)
胱天蛋白酶10(CASP10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Caspase 10 (CASP10)48T/96TY09089Homo sapiens (Human)
硫氧还蛋白结合蛋白2(TBP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Thioredoxin Binding Protein 2 (TBP2)48T/96TY09090Homo sapiens (Human)
发动蛋白2(DNM2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dynamin 2 (DNM2)48T/96TY09091Homo sapiens (Human)
磷脂爬行酶2(PLSCR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipid Scramblase 2 (PLSCR2)48T/96TY09092Homo sapiens (Human)
狼疮脑抗原(LBA1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lupus Brain Antigen 1 (LBA1)48T/96TY09093Homo sapiens (Human)
VGF神经生长因子诱导蛋白(VGF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for VGF Nerve Growth Factor Inducible (VGF)48T/96TY09094Homo sapiens (Human)
雄激素诱导蛋白1(AIG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Androgen Induced Protein 1 (AIG1)48T/96TY09095Homo sapiens (Human)
214kDa核孔蛋白(NUP214)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nucleoporin 214kDa (NUP214)48T/96TY09096Homo sapiens (Human)
免疫抑制酸性蛋白(IAP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Immunosuppressive Acidic Protein (IAP)48T/96TY09097Homo sapiens (Human)
抗内因子抗体(AIFA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Anti-Intrinsic Factor Antibody (AIFA)48T/96TY09098Homo sapiens (Human)
脑啡肽(ENK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Enkephalin (ENK)48T/96TY09099Homo sapiens (Human)
低分子量激肽原(LMWK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Low Molecular Weight Kininogen (LMWK)48T/96TY09100Homo sapiens (Human)
205kDa核孔蛋白(NUP205)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nucleoporin 205kDa (NUP205)48T/96TY09101Homo sapiens (Human)
簇集蛋白(CLU)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Clusterin (CLU)48T/96TY09102Homo sapiens (Human)
抗原提呈关联转运蛋白(TAP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transporter Associated With Antigen Processing (TAP)48T/96TY09103Homo sapiens (Human)
188kDa核孔蛋白(NUP188)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nucleoporin 188kDa (NUP188)48T/96TY09104Homo sapiens (Human)
皮肤T-淋巴细胞关联抗原(CTAGE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cutaneous T-Cell Lymphocyte Associated Antigen (CTAGE)48T/96TY09105Homo sapiens (Human)
葡萄糖转运蛋白1(GLUT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucose Transporter 1 (GLUT1)48T/96TY09106Homo sapiens (Human)
神经激肽B(NKB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neurokinin B (NKB)48T/96TY09107Homo sapiens (Human)
神经降压素(NT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neurotensin (NT)48T/96TY09108Homo sapiens (Human)
接触蛋白2(CNTN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Contactin 2 (CNTN2)48T/96TY09109Homo sapiens (Human)
肾上腺髓质素(ADM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Adrenomedullin (ADM)48T/96TY09110Homo sapiens (Human)
丝裂原激活蛋白激酶14(MAPK14)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mitogen Activated Protein Kinase 14 (MAPK14)48T/96TY09111Homo sapiens (Human)
生长激素诱导跨膜蛋白(GHITM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Growth Hormone Inducible Transmembrane Protein (GHITM)48T/96TY09112Homo sapiens (Human)
接触蛋白4(CNTN4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Contactin 4 (CNTN4)48T/96TY09113Homo sapiens (Human)
颗粒酶K(GZMK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Granzyme K (GZMK)48T/96TY09114Mus musculus (Mouse)
视网膜母细胞瘤蛋白1(RB1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Retinoblastoma Protein 1 (RB1)48T/96TY09115Homo sapiens (Human)
嗜铬蛋白A(CHGA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Chromogranin A (CHGA)48T/96TY09116Homo sapiens (Human)
毒蕈碱型胆碱受体M2(CHRM2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cholinergic Receptor, Muscarinic 2 (CHRM2)48T/96TY09117Homo sapiens (Human)
缓激肽(BK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bradykinin (BK)48T/96TY09118Homo sapiens (Human)
纤维蛋白原β(FGβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibrinogen Beta (FGb)48T/96TY09119Homo sapiens (Human)
松弛肽(RLN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Relaxin (RLN)48T/96TY09120Homo sapiens (Human)
TGFβ诱导早期应答基因1(TIEG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for TGF Beta Inducible Early Response Gene 1 (TIEG1)48T/96TY09121Rattus norvegicus (Rat)
激素脱落酸(ABA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Abscisic Acid (ABA)48T/96TY09122General
胎盘碱性磷酸酶(ALPP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alkaline Phosphatase, Placental (ALPP)48T/96TY09123Homo sapiens (Human)
接触蛋白关联蛋白样蛋白4(CNTNAP4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Contactin Associated Protein Like Protein 4 (CNTNAP4)48T/96TY09124Homo sapiens (Human)
糖抗原50(CA50)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Carbohydrate Antigen 50 (CA50)48T/96TY09125Homo sapiens (Human)
突触核蛋白α(SNCα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Synuclein Alpha (SNCa)48T/96TY09126Homo sapiens (Human)
神经细胞黏附分子(NCAM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neural Cell Adhesion Molecule (NCAM)48T/96TY09127Homo sapiens (Human)
抗补体1q抗体(Anti-C1q)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Anti-Complement 1q Antibody (Anti-C1q)48T/96TY09128Homo sapiens (Human)
胃泌素(GT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Gastrin (GT)48T/96TY09129Homo sapiens (Human)
毒蕈碱型胆碱受体M4(CHRM4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cholinergic Receptor, Muscarinic 4 (CHRM4)48T/96TY09130Homo sapiens (Human)
MAX基因关联蛋白A(MgA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for MAX Gene Associated Protein (MgA)48T/96TY09131Homo sapiens (Human)
细胞毒性T-淋巴细胞关联抗原4(CTLA4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4)48T/96TY09132Homo sapiens (Human)
角蛋白18(KRT18)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Keratin 18 (KRT18)48T/96TY09133Homo sapiens (Human) 

细胞外基质磷酸糖蛋白(MEPE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Extracellular Phosphoglycoprotein (MEPE)48T/96TY09134Homo sapiens (Human)

 

各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。

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