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人沙眼衣原体酶免试剂盒,(CT)ELISA检测试剂盒
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更新时间:2016-10-03  |  阅读:1143

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人沙眼衣原体酶免试剂盒,(CT)ELISA检测试剂盒,英文名:CT ELISA Kit,英文缩写:CT ,常见试剂盒规格:96T/48T,产品别名:人沙眼衣原体ELISA检测试剂盒,CT 检测试剂盒,CT 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人沙眼衣原体酶免试剂盒,(CT)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重

凋亡蛋白酶激活因子1(APAF1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1)48T/96TY08985Homo sapiens (Human)
V-Myb髓细胞组织增生病毒癌基因同源物(MYB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for V-Myb Myeloblastosis Viral Oncogene Homolog (MYB)48T/96TY08986Homo sapiens (Human)
70kDa热休克蛋白4(HSPA4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock 70kDa Protein 4 (HSPA4)48T/96TY08987Homo sapiens (Human)
泛素连接酶E3A(UBE3A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ubiquitin Protein Ligase E3A (UBE3A)48T/96TY08988Homo sapiens (Human)
多聚免疫球蛋白受体(PIGR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Polymeric Immunoglobulin Receptor (PIGR)48T/96TY08989Homo sapiens (Human)
促胰液素(SCT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secretin (SCT)48T/96TY08990Homo sapiens (Human)
促胰液素受体(SCTR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secretin Receptor (SCTR)48T/96TY08991Homo sapiens (Human)
ⅡD组磷脂酶A2(PLA2G2D)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipase A2, Group IID (PLA2G2D)48T/96TY08992Homo sapiens (Human)
S100钙结合蛋白A13(S100A13)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for S100 Calcium Binding Protein A13 (S100A13)48T/96TY08993Homo sapiens (Human)
通用转录因子ⅡH肽1(GTF2H1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for General Transcription Factor IIH, Polypeptide 1 (GTF2H1)48T/96TY08994Homo sapiens (Human)
S100钙结合蛋白A12(S100A12)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for S100 Calcium Binding Protein A12 (S100A12)48T/96TY08995Homo sapiens (Human)
70kDa热休克蛋白1A(HSPA1A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock 70kDa Protein 1A (HSPA1A)48T/96TY08996Homo sapiens (Human)
70kDa热休克蛋白9(HSPA9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock 70kDa Protein 9 (HSPA9)48T/96TY08997Homo sapiens (Human)
甘丙肽(GAL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Galanin (GAL)48T/96TY08998Homo sapiens (Human)
线粒体超氧化物歧化酶(SOD2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Superoxide Dismutase 2, Mitochondrial (SOD2)48T/96TY08999Homo sapiens (Human)
肠碱性磷酸酶(ALPI)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alkaline Phosphatase, Intestinal (ALPI)48T/96TY09000Homo sapiens (Human)
早期生长应答因子4(EGR4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Early Growth Response Protein 4 (EGR4)48T/96TY09001Homo sapiens (Human)
35kDa核孔蛋白(NUP35)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nucleoporin 35kDa (NUP35)48T/96TY09002Homo sapiens (Human)
37kDa核孔蛋白(NUP37)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nucleoporin 37kDa (NUP37)48T/96TY09003Homo sapiens (Human)
谷胱甘肽S转移酶κ1(GSTκ1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutathione S Transferase Kappa 1 (GSTk1)48T/96TY09004Homo sapiens (Human)
谷胱甘肽S转移酶π1(GSTπ1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutathione S Transferase Pi (GSTp)48T/96TY09005Homo sapiens (Human)
肝肾骨碱性磷酸酶(ALPL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alkaline Phosphatase, Liver/Bone/Kidney (ALPL)48T/96TY09006Homo sapiens (Human)
骨涎蛋白(BSP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Sialoprotein (BSP)48T/96TY09007Homo sapiens (Human)
凝血因子ⅩⅢA1肽(F13A1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Coagulation Factor XIII A1 Polypeptide (F13A1)48T/96TY09008Homo sapiens (Human)
脑指蛋白(BFP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Brain Finger Protein (BFP)48T/96TY09009Homo sapiens (Human)
核基质蛋白4(NMP4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nuclear Matrix Protein 4 (NMP4)48T/96TY09010Homo sapiens (Human)
胎儿肾钙黏蛋白(KCAD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cadherin, Fetal Kidney (KCAD)48T/96TY09011Homo sapiens (Human)
花生四烯酸(AA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Arachidonic Acid (AA)48T/96TY09012General
低密度脂蛋白(LDL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Low Density Lipoprotein (LDL)48T/96TY09013Homo sapiens (Human)
肌细胞生成素(MYOG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myogenin (MYOG)48T/96TY09014Homo sapiens (Human)
85kDa核孔蛋白(NUP85)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nucleoporin 85kDa (NUP85)48T/96TY09015Homo sapiens (Human)
107kDa核孔蛋白(NUP107)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nucleoporin 107kDa (NUP107)48T/96TY09016Homo sapiens (Human)
中性粒细胞碱性磷酸酶(NAP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neutrophil Alkaline Phosphatase (NAP)48T/96TY09017Homo sapiens (Human)
甲胎蛋白异质体1(αFPL1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-Fetoprotein Lens Culinaris Agglutinin 1 (aFPL1)48T/96TY09018Homo sapiens (Human)
甲胎蛋白异质体2(αFPL2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-Fetoprotein Lens Culinaris Agglutinin 2 (aFPL2)48T/96TY09019Homo sapiens (Human)
成纤维细胞生长因子23(FGF23)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Growth Factor 23 (FGF23)48T/96TY09020Homo sapiens (Human)
CCAAT增强子结合蛋白γ(CEBPγ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for CCAAT/Enhancer Binding Protein Gamma (CEBPg)48T/96TY09021Homo sapiens (Human)
补体1q(C1q)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Complement 1q (C1q)48T/96TY09022Homo sapiens (Human)
凝血因子Ⅴ(F5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Coagulation Factor V (F5)48T/96TY09023Homo sapiens (Human)
凝血因子Ⅴ(F5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Coagulation Factor V (F5)48T/96TY09024Rattus norvegicus (Rat)
凝血因子ⅩⅢ(F13)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Coagulation Factor XIII (F13)48T/96TY09025Homo sapiens (Human)
凝血因子ⅩⅢ(F13)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Coagulation Factor XIII (F13)48T/96TY09026Rattus norvegicus (Rat)
凝血因子Ⅻ(F12)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Coagulation Factor XII (F12)48T/96TY09027Homo sapiens (Human)
凝血因子Ⅻ(F12)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Coagulation Factor XII (F12)48T/96TY09028Rattus norvegicus (Rat)
免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Immunoglobulin G (IgG)48T/96TY09029Cavia (Guinea pig )
三叶因子2(TFF2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Trefoil Factor 2 (TFF2)48T/96TY09030Homo sapiens (Human)
三叶因子2(TFF2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Trefoil Factor 2 (TFF2)48T/96TY09031Sus scrofa; Porcine (Pig)
胆囊收缩素(CCK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cholecystokinin (CCK)48T/96TY09032Sus scrofa; Porcine (Pig)
二胺氧化酶(DAO)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Diamine Oxidase (DAO)48T/96TY09033Sus scrofa; Porcine (Pig)
表面活性物质关联蛋白B(SPB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Surfactant Associated Protein B (SPB)48T/96TY09034Homo sapiens (Human) 

各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。

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