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人抗核周因子抗体酶免试剂盒,(APF)ELISA检测试剂盒,英文名:APF ELISA Kit,英文缩写:APF ,常见试剂盒规格:96T/48T,产品别名:人抗核周因子抗体ELISA检测试剂盒,APF 检测试剂盒,APF 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人抗核周因子抗体酶免试剂盒,(APF)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重
蛋白激酶抑制因子γ(PKIγ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Kinase Inhibitor Gamma (PKIg)48T/96TY08685Mus musculus (Mouse)
血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)48T/96TY08686Oryctolagus cuniculus (Rabbit)
血管性血友病因子(vWF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Von Willebrand Factor (vWF)48T/96TY08687Oryctolagus cuniculus (Rabbit)
血管紧张素Ⅰ(AngⅠ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiotensin I (AngI)48T/96TY08688Homo sapiens (Human)
血管紧张素Ⅰ(AngⅠ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiotensin I (AngI)48T/96TY08689Rattus norvegicus (Rat)
血管紧张素Ⅰ(AngⅠ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiotensin I (AngI)48T/96TY08690Mus musculus (Mouse)
细胞色素C(CYCS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytochrome C, Somatic (CYCS)48T/96TY08691Homo sapiens (Human)
细胞色素C(CYCS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytochrome C, Somatic (CYCS)48T/96TY08692Rattus norvegicus (Rat)
细胞色素C(CYCS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytochrome C, Somatic (CYCS)48T/96TY08693Mus musculus (Mouse)
白三烯C4(LTC4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leukotriene C4 (LTC4)48T/96TY08694General
胰淀素(IAPP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Islet Amyloid Polypeptide (IAPP)48T/96TY08695Homo sapiens (Human)
胰淀素(IAPP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Islet Amyloid Polypeptide (IAPP)48T/96TY08696Rattus norvegicus (Rat)
胰淀素(IAPP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Islet Amyloid Polypeptide (IAPP)48T/96TY08697Mus musculus (Mouse)
低氧诱导因子1α(HIF1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)48T/96TY08698Homo sapiens (Human)
低氧诱导因子1α(HIF1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)48T/96TY08699Rattus norvegicus (Rat)
低氧诱导因子1α(HIF1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)48T/96TY08700Mus musculus (Mouse)
B-淋巴细胞激活抗原B7-2(LAB7-2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for B-Lymphocyte Activation Antigen B7-2 (LAB7-2)48T/96TY08701Homo sapiens (Human)
B-淋巴细胞激活抗原B7-2(LAB7-2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for B-Lymphocyte Activation Antigen B7-2 (LAB7-2)48T/96TY08702Rattus norvegicus (Rat)
B-淋巴细胞激活抗原B7-2(LAB7-2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for B-Lymphocyte Activation Antigen B7-2 (LAB7-2)48T/96TY08703Mus musculus (Mouse)
白介素6(IL6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 6 (IL6)48T/96TY08704Chicken (Gallus)
干扰素γ(IFNγ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Gamma (IFNg)48T/96TY08705Chicken (Gallus)
90kDa热休克蛋白β1(HSP90β1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1)48T/96TY08706Homo sapiens (Human)
90kDa热休克蛋白β1(HSP90β1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1)48T/96TY08707Rattus norvegicus (Rat)
90kDa热休克蛋白β1(HSP90β1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1)48T/96TY08708Mus musculus (Mouse)
超氧化物歧化酶(SOD)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Superoxide Dismutases (SOD)48T/96TY08709Homo sapiens (Human)
超氧化物歧化酶1(SOD1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Superoxide Dismutase 1, Soluble (SOD1)48T/96TY08710Rattus norvegicus (Rat)
超氧化物歧化酶1(SOD1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Superoxide Dismutase 1, Soluble (SOD1)48T/96TY08711Mus musculus (Mouse)
丙二醛(MDA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Malondialdehyde (MDA)48T/96TY08712General
60kD热休克蛋白(HSPD1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock 60kD Protein 1, Chaperonin (HSPD1)48T/96TY08713Homo sapiens (Human)
60kD热休克蛋白(HSPD1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock 60kD Protein 1, Chaperonin (HSPD1)48T/96TY08714Rattus norvegicus (Rat)
60kD热休克蛋白(HSPD1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock 60kD Protein 1, Chaperonin (HSPD1)48T/96TY08715Mus musculus (Mouse)
血管紧张素原(AGT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiotensinogen (AGT)48T/96TY08716Homo sapiens (Human)
血管紧张素原(AGT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiotensinogen (AGT)48T/96TY08717Rattus norvegicus (Rat)
血管紧张素原(AGT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiotensinogen (AGT)48T/96TY08718Mus musculus (Mouse)
脂质转运蛋白(CETP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cholesteryl Ester Transfer Protein (CETP)48T/96TY08719Homo sapiens (Human)
神经型一氧化氮合酶()检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase 1, Neuronal ()48T/96TY08720Homo sapiens (Human)
神经型一氧化氮合酶()检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase 1, Neuronal ()48T/96TY08721Mus musculus (Mouse)
神经型一氧化氮合酶()检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase 1, Neuronal ()48T/96TY08722Rattus norvegicus (Rat)
内皮型一氧化氮合酶(NOS3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3)48T/96TY08723Sus scrofa; Porcine (Pig)
内皮型一氧化氮合酶(NOS3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3)48T/96TY08724Cavia (Guinea pig )
C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for C Reactive Protein (CRP)48T/96TY08725Bos taurus; Bovine (Cattle)
α1-酸性糖蛋白(α1AGP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)48T/96TY08726Homo sapiens (Human)
α1-酸性糖蛋白(α1AGP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)48T/96TY08727Bos taurus; Bovine (Cattle)
α1-酸性糖蛋白(α1AGP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)48T/96TY08728Rattus norvegicus (Rat)
α1-酸性糖蛋白(α1AGP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)48T/96TY08729Mus musculus (Mouse)
铜蓝蛋白(CP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ceruloplasmin (CP)48T/96TY08730Rattus norvegicus (Rat)
结合珠蛋白(Hpt)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Haptoglobin (Hpt)48T/96TY08731Homo sapiens (Human)
结合珠蛋白(Hpt)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Haptoglobin (Hpt)48T/96TY08732Bos taurus; Bovine (Cattle)
结合珠蛋白(Hpt)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Haptoglobin (Hpt)48T/96T Y08733Mus musculus (Mouse)
结合珠蛋白(Hpt)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Haptoglobin (Hpt)48T/96TY08734Rattus norvegicus (Rat)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。