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人抗胰岛素受体抗体酶免试剂盒,(AIRA)ELISA检测试剂盒 ,英文名:AIRA ELISA Kit ,英文缩写:AIRA ,常见试剂盒规格:96T/48T,产品别名:人抗胰岛素受体抗体ELISA检测试剂盒,AIRA 检测试剂盒, AIRA 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人抗胰岛素受体抗体酶免试剂盒,(AIRA)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重
淀粉样蛋白β1-40(Aβ1-40)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Amyloid Beta Peptide 1-40 (Ab1-40)48T/96TY08485Mus musculus (Mouse)
淀粉样蛋白β1-40(Aβ1-40)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Amyloid Beta Peptide 1-40 (Ab1-40)48T/96TY08486Rattus norvegicus (Rat)
热休克蛋白β2(HSPβ2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock Protein Beta 2 (HSPb2)48T/96TY08487Mus musculus (Mouse)
热休克蛋白β2(HSPβ2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock Protein Beta 2 (HSPb2)48T/96TY08488Rattus norvegicus (Rat)
热休克蛋白β2(HSPβ2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock Protein Beta 2 (HSPb2)48T/96TY08489Oryctolagus cuniculus (Rabbit)
热休克蛋白40(HSP40)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock Protein 40 (HSP40)48T/96TY08490Rattus norvegicus (Rat)
热休克蛋白40(HSP40)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock Protein 40 (HSP40)48T/96TY08491Mus musculus (Mouse)
热休克蛋白40(HSP40)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock Protein 40 (HSP40)48T/96TY08492Xenopus (Clawed frog)
90kDa热休克蛋白β1(HSP90β1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1)48T/96TY08493Oryctolagus cuniculus (Rabbit)
70kDa热休克蛋白1A(HSPA1A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock 70kDa Protein 1A (HSPA1A)48T/96TY08494Sus scrofa; Porcine (Pig)
90kDa热休克蛋白β1(HSP90β1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1)48T/96TY08495Sus scrofa; Porcine (Pig)
脂蛋白关联磷脂酶A2(LpPLA2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipase A2, Lipoprotein Associated (LpPLA2)48T/96TY08496Homo sapiens (Human)
脂蛋白关联磷脂酶A2(LpPLA2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipase A2, Lipoprotein Associated (LpPLA2)48T/96TY08497Rattus norvegicus (Rat)
脂蛋白关联磷脂酶A2(LpPLA2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipase A2, Lipoprotein Associated (LpPLA2)48T/96TY08498Mus musculus (Mouse)
内皮型一氧化氮合酶(NOS3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3)48T/96TY08499Homo sapiens (Human)
内皮型一氧化氮合酶(NOS3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3)48T/96TY08500Rattus norvegicus (Rat)
内皮型一氧化氮合酶(NOS3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3)48T/96TY08501Mus musculus (Mouse)
诱导型一氧化氮合酶(NOS2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2)48T/96TY08502Rattus norvegicus (Rat)
诱导型一氧化氮合酶(NOS2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2)48T/96TY08503Mus musculus (Mouse)
诱导型一氧化氮合酶(NOS2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2)48T/96TY08504Oryctolagus cuniculus (Rabbit)
一氧化氮合酶运输因子(NOSTRIN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase Trafficker (NOSTRIN)48T/96TY08505Rattus norvegicus (Rat)
一氧化氮合酶运输因子(NOSTRIN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nitric Oxide Synthase Trafficker (NOSTRIN)48T/96TY08506Mus musculus (Mouse)
巨噬细胞移动抑制因子(MIF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Macrophage Migration Inhibitory Factor (MIF)48T/96TY08507Homo sapiens (Human)
趋化因子C-C-基元受体8(CCR8)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Chemokine C-C-Motif Receptor 8 (CCR8)48T/96TY08508Mus musculus (Mouse)
免疫球蛋白G1(IgG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Immunoglobulin G1 (IgG1)48T/96TY08509Homo sapiens (Human)
环磷酸鸟苷(cGMP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cyclic Guanosine Monophosphate (cGMP)48T/96TY08510General
肿瘤蛋白p53(TP53)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tumor Protein p53 (TP53)48T/96TY08511Homo sapiens (Human)
肿瘤蛋白p53(TP53)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tumor Protein p53 (TP53)48T/96TY08512Mus musculus (Mouse)
肿瘤蛋白p53(TP53)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tumor Protein p53 (TP53)48T/96TY08513Rattus norvegicus (Rat)
P物质(SP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Substance P (SP)48T/96TY08514Rattus norvegicus (Rat)
P物质(SP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Substance P (SP)48T/96TY08515Mus musculus (Mouse)
速激肽受体1(TACR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tachykinin Receptor 1 (TACR1)48T/96TY08516Homo sapiens (Human)
速激肽受体1(TACR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tachykinin Receptor 1 (TACR1)48T/96TY08517Rattus norvegicus (Rat)
速激肽受体1(TACR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tachykinin Receptor 1 (TACR1)48T/96TY08518Mus musculus (Mouse)
5-羟色胺(5-HT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for 5-Hydroxytryptamine (5-HT)48T/96TY08519General
γ-氨基丁酸(γABA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Gamma-Aminobutyric Acid (gABA)48T/96TY08520General
多巴胺受体D4(DRD4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dopamine Receptor D4 (DRD4)48T/96TY08521Homo sapiens (Human)
多巴胺受体D4(DRD4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dopamine Receptor D4 (DRD4)48T/96TY08522Rattus norvegicus (Rat)
α2-纤溶酶抑制因子(α2PI)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Alpha-2-Plasmin Inhibitor (a2PI)48T/96TY08523Rattus norvegicus (Rat)
多巴胺受体D4(DRD4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dopamine Receptor D4 (DRD4)48T/96TY08524Mus musculus (Mouse)
多巴胺受体D4(DRD4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dopamine Receptor D4 (DRD4)48T/96TY08525Canis familiaris; Canine (Dog)
M2-型丙酮酸激酶(PKM2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pyruvate Kinase, Muscle (PKM2)48T/96TY08526Homo sapiens (Human)
M2-型丙酮酸激酶(PKM2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pyruvate Kinase, Muscle (PKM2)48T/96TY08527Rattus norvegicus (Rat)
M2-型丙酮酸激酶(PKM2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pyruvate Kinase, Muscle (PKM2)48T/96TY08528Mus musculus (Mouse)
凝血因子Ⅱ(F2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Coagulation Factor II (F2)48T/96TY08529Rattus norvegicus (Rat)
凝血因子Ⅱ(F2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Coagulation Factor II (F2)48T/96TY08530Mus musculus (Mouse)
前动力蛋白受体2(PKR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prokineticin Receptor 2 (PKR2)48T/96TY08531Homo sapiens (Human)
牛血清白蛋白(BSA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bovine Serum Albumin (BSA)48T/96TY08532General
免疫球蛋白G3(IgG3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Immunoglobulin G3 (IgG3)48T/96TY08533Mus musculus (Mouse)
干扰素α(IFNα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Alpha (IFNa)48T/96TY08534Homo sapiens (Human)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。