大鼠脂联素(ADP)ELISA试剂盒 Rat adiponectin,ADP ELISA Kit ,规格:96T/48T,产品别名: 大鼠脂联素(ADP)检测试剂盒
大鼠脂联素(ADP)ELISA试剂盒原理的简要说明书:Adiponectin(又名Acrp30、AdipoQ、OBG3、apm1等),脂联素。Adiponectin是近年发现的一种重要的脂肪细胞因子,Adiponectin由244个氨基酸组成,包括由1-17个氨基酸组成的信号肽、18-41位氨基酸组成的non-homologous区、42-107位氨基酸组成的胶原区和108-244位氨基酸组成的球状结构域(gAcrp30)。Acrp30从脂肪细胞表达分泌后主要存在于外周血液中,Acrp30 水平与体重相关。
E-(Hu)(07)-01053 人嗜酸性白血球相关之RNA水解酵素家族成员3(EAR3)酶联免疫试剂盒 ,英文名: eosinophil-associated ribonuclease A family member 3,EAR3 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01054 人嗜酸性白血球相关之RNA水解酵素家族成员2(EAR2)酶联免疫试剂盒 ,英文名: eosinophil-associated ribonuclease A family member 2 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01055 人二氢嘧啶酶样3(DPYSL3)酶联免疫试剂盒 ,英文名: dihydropyrimidinase-like 3,DPYSL3 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01056 人色氨酰tRNA合成酶(WARS)酶联免疫试剂盒 ,英文名: tryptophanyl-tRNA synthetase,WARS ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01057 人己糖激酶3(HK3)酶联免疫试剂盒 ,英文名: hexokinase 3,HK3 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01058 人血小板型磷酸果糖激酶(PFKP)酶联免疫试剂盒 ,英文名: phosphofructokinase-plaet,PFKP ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01059 人尿苷二磷酸葡萄糖神经酰铵葡萄糖基转移酶(UGCG)酶联免疫试剂盒 ,英文名: UDP-glucose ceramide glucosyltransferase ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01060 人凋亡相关半胱氨酸肽酶4(Casp4)酶联免疫试剂盒 ,英文名: caspase 4-apoptosis-related cysteine peptidase,Casp4 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01061 人吡哆醛/吡哆醇维生素B6激酶(PDXK)酶联免疫试剂盒 ,英文名: caspase 4-apoptosis-related cysteine peptidase,Casp4 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01062 人二氢嘧啶酶样2(DPYSL2)酶联免疫试剂盒 ,英文名: dihydropyrimidinase-like 2,DPYSL2 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01063 人血清/糖皮质激素调节激酶2(GSK2)酶联免疫试剂盒 ,英文名: serum/glucocorticoid regulated kinase 2,GSK2 ISA KIT ,规格: 96T/48T
E-(Hu)(07)-01064 人丝裂原活化蛋白激酶激酶6(MKK6)酶联免疫试剂盒 ,英文名: mitogen activated protein kinase kinase 6,MKK6 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01065 人吡哆醛/吡哆醇维生素B6磷酸酶(PDXP)酶联免疫试剂盒 ,英文名: pyridoxal/pyridoxine vitamin B6-phosphatase,PDXP ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01066 人T细胞特异性鸟苷三磷酸酶(Tgtp)酶联免疫试剂盒 ,英文名: T-cell specific GTPase,Tgtp ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01067 人法尼基二磷酸法尼基转移酶1(FDFT1)酶联免疫试剂盒 ,英文名: farnesyl-diphosphate farnesyltransferase 1,FDFT1 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01068 人D-乳酸脱氢酶(D-LDH) 酶联免疫试剂盒 ,英文名: D-Lactate Dehydrogenase,D-LDH ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01069 人α1抗胰蛋白酶(α1-AT)酶联免疫试剂盒 ,英文名: α1-Antitrypsin,α1-AT ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01070 人激肽释放酶1(KLK 1)酶联免疫试剂盒 ,英文名: Kallikrein 1,KLK 1 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01071 人磷酸化乙酰辅酶A(PaCoA)酶联免疫试剂盒 ,英文名: Phosphorylated acetyl CoA,PaCoA ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01072 人磷酸甘油酸变位酶2(PGAM2)酶联免疫试剂盒 ,英文名: phosphoglyceRate mutase 2,PGAM2 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01073 人前列腺素D合成酶(PTGDS)酶联免疫试剂盒 ,英文名: Prostaglandin D synthase,PTGDS ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01074 人肽酰脯氨酰异构酶(亲环蛋白)样1(PPIL1)酶联免疫试剂盒 ,英文名: peptidylprolyl isomerase(cyclophilin)-like 1,PPIL1 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01075 人促分裂素原活化蛋白激酶激活的蛋白激酶3(MAPKAPK3)酶联免疫试剂盒 ,英文名: mitogen-activated protein kinase-activated protein kinase 3,MAPKAPK3 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01076 人血管紧张素Ⅰ转化酶(ACEⅠ)酶联免疫试剂盒 ,英文名: Angiotensin Ⅰ Converting Enzyme,ACEⅠ ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01077 人胱天蛋白酶12(Casp-12)酶联免疫试剂盒 ,英文名: Caspase 12,Casp-12 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01078 人蛋白激酶A(PKA)酶联免疫试剂盒 ,英文名: Protein kinase A,PKA ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01079 人解整合素样金属蛋白酶10(ADAM10)酶联免疫试剂盒 ,英文名: A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01080 人胸苷酸合成酶(TS)酶联免疫试剂盒 ,英文名: thymidylate synthetase,TS ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01081 人胸腺嘧啶核苷磷酸化酶(TP)酶联免疫试剂盒 ,英文名: thymidinephosphorylase,TP ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01082 人多聚ADP核糖聚合酶(PARP)酶联免疫试剂盒 ,英文名: Poly ADP ribose polymerase,PARP ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01083 人葡萄糖激酶(GCK)酶联免疫试剂盒 ,英文名: glucokinase,GCK ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01084 人单铵氧化酶(MAO)酶联免疫试剂盒 ,英文名: monoamine oxidase,MAO ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01085 人胞浆型磷脂酶A2(cPLA2)酶联免疫试剂盒 ,英文名: cytosolic phospholipase A2,cPLA2 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01086 人二铵氧化酶(DAO)酶联免疫试剂盒 ,英文名: diamine oxidase,DAO ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01087 人环加氧酶2(COX-2)酶联免疫试剂盒 ,英文名: cyclooxygenase-2,COX-2 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01088 人周期素依赖性激酶5(CDK5)酶联免疫试剂盒 ,英文名: Cyclin Dependent Kinase 5,CDK5 ELISA Kit ,大鼠脂联素(ADP)ELISA试剂盒规格: 96T/48T
E-(Hu)(07)-01089 人激肽释放酶10(KLK 10)酶联免疫试剂盒 ,英文名: Kallikrein 10,KLK 10 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01090 人磷酯酶Cγ链(PLCγ1)酶联免疫试剂盒 ,英文名: Phospholipase Cγ1,PLCγ1 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01091 人神经氨酸酶(NA)酶联免疫试剂盒 ,英文名: neuraminidase,NA ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01092 人磷酯酰肌醇特异性磷酯酶C(PIPLC)酶联免疫试剂盒 ,英文名: phosphoinositide-specific phospholipase C,PIPLC ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01093 人酪氨酸激酶2(Tyk-2)酶联免疫试剂盒 ,英文名: tyrosine kinase2,Tyk-2 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01094 人白介素1β转换酶(ICE)酶联免疫试剂盒 ,英文名: Interleukin 1β converting enzyme,ICE ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01095 人整合素连接激酶1(ILK-1)酶联免疫试剂盒 ,英文名: integrin-linked kinase 1,ILK-1 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01096 人细胞色素P450c21B/21-羟化酶(CYP21B)酶联免疫试剂盒 ,英文名: Cytochrome P450c21/21-hydroxylase,CYP21B ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01097 人细胞色素P450c21A/21-羟化酶(CYP21A)酶联免疫试剂盒 ,英文名: Cytochrome P450c21/21-hydroxylase,CYP21A ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01098 人末端脱氧核苷酸转移酶(TdT)酶联免疫试剂盒 ,英文名: terminal deoxynucleotidyl transferase,TdT ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01099 人吲哚铵2,3-双加氧酶(IDO)酶联免疫试剂盒 ,英文名: indoleamine 2,3-dioxygenase,IDO ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01100 人脾脏酪氨酸激酶(Syk)酶联免疫试剂盒 ,英文名: spleen tyrosine kinase,Syk ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01101 人丝氨酸/苏氨酸蛋白磷酸酶(STK)酶联免疫试剂盒 ,英文名: serine/threonine protein kinase,STK ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01102 人白介素受体相关激酶(IRAK)酶联免疫试剂盒 ,英文名: interleukin receptor associated kinase,IRAK ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01103 人磷酸烯醇式丙酮酸羧激酶(PCK)酶联免疫试剂盒 ,英文名: phosphoenolpyruvate carboxykinase,PCK ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01104 人胰蛋白酶(trypsin)酶联免疫试剂盒 ,英文名: trypsin ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01105 人β位淀粉样前体蛋白裂解酶1(BACE1)酶联免疫试剂盒 ,英文名: β-site APP-Cleaving Enzyme 1,1BACE1 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01106 人蛋白二硫化物异构酶前体(PDI)酶联免疫试剂盒 ,英文名: Protein disulfide-isomerase precursor,PDI ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01107 人蛋白二硫化物异构酶A3(PDIA3)酶联免疫试剂盒 ,英文名: protein disulfide-isomerase A3,PDIA3 ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01108 人山梨醇脱氢酶(SDH)酶联免疫试剂盒 ,英文名: sorbitol dehydrogenase,SDH ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01109 人肽基脯氨酰顺反异构酶(PPI)酶联免疫试剂盒 ,英文名: Peptidyl prolyl cis/trans isomerase,PPI ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01110 人鸟氨酸氨基甲酰转移酶(OCT)酶联免疫试剂盒 ,英文名: ornithine carbamoyl transferase,OCT ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01111 人内脏脂肪特异性丝氨酸蛋白酶抑制剂(vaspin)酶联免疫试剂盒 ,英文名: Visceral adipose-specific serine protease inhibitor,vaspin ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01112 人蛋白磷酸酶(PP)酶联免疫试剂盒 ,英文名: Protein Phosphatase,PP ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01113 人硫氧还蛋白还原酶(TrxR)酶联免疫试剂盒 ,英文名: thioredoxin reductase,TrxR ELISA Kit ,规格: 96T/48T
E-(Hu)(07)-01114 人天青杀素(AZU)酶联免疫试剂盒 ,英文名: azurocidin,AZU ELISA Kit ,规格: 96T/48T
大鼠脂联素(ADP)ELISA试剂盒操作步骤
1.使用前,将所有试剂充分混匀。不要使液体产生大量的泡沫,以免加样时加入大量的气泡,产生加样上的误差。
2.根据待测样品数量加上标准品的数量决定所需的板条数。每个标准品和空白孔建议做复孔。每个样品根据自己的数量来定,能使用复孔的尽量做复孔。标本用标本稀释液1:1稀释后加入50ul于反应孔内。
3.加入稀释好后的标准品50ul于反应孔、加入待测样品50ul于反应孔内。立即加入50ul的生物素标记的抗体。盖上膜板,轻轻振荡混匀,37℃温育1小时。
4.甩去孔内液体,每孔加满洗涤液,振荡30秒,甩去洗涤液,用吸水纸拍干。重复此操作3次。如果用洗板机洗涤,洗涤次数增加一次。
5.每孔加入80ul的亲和链酶素-HRP,轻轻振荡混匀,37℃温育30分钟。
6.甩去孔内液体,每孔加满洗涤液,振荡30秒,甩去洗涤液,用吸水纸拍干。重复此操作3次。如果用洗板机洗涤,洗涤次数增加一次。
7.每孔加入底物A、B各50ul,轻轻振荡混匀,37℃温育10分钟。避免光照。
8.取出酶标板,迅速加入50ul终止液,加入终止液后应立即测定结果。
9.在450nm波长处测定各孔的OD值。